Fast Hydrogen Peroxide Assay

Description

Hydrogen Peroxide (H2O2) is a reactive oxygen metabolic byproduct that serves as a key regulator for a number of oxidative stress-related states. It is involved in many pathological processes of diseases such as asthma, inflammatory arthritis, atherosclerosis, diabetic vasculopathy, osteoporosis, neuro-degenerative diseases, Down’s syndrome, and immune system diseases.

Tribioscience’s ADHP-red Hydrogen Peroxide Assay Kit is a sensitive, simple, direct, and HTS-ready colorimetric/fluorometric assay designed to measure H2O2 levels in biological samples. In the presence of Horse Radish Peroxidase (HRP), the ADHP-red probe reacts with H2O2 to produce a resorufin that appears red in color. The resorufin can be measured with the absorption of 570nm (λmax = 570nm) for colorimetric or a highly stable fluorometric assay at maximum emission of 585nm (Ex/Em = 535/587 nm).

Applications:

• Detection of HRP activity on liquid systems such as ELISA.
• Western Blot or other immunoassays.
• Custom packaging and bulk purchase information are available upon request.

 

Key Features:

• Flexible: suitable for colorimetric and fluorometric methods.
• Accurate: Use 50 µL samples. Detection ranges 0.4-200 µM in 96-well plate for colorimetric assay and fluorometric assay.
• Simple and high-throughput: One-step procedure – Just load-incubate-read. The kit can be used for a robust method.
• Time-saving less than 30 minutes.

Kit Contents:

Component	100x Rxns	200x Rxns
ADHP Solution	60μL	120μL
H2O2 Standard (0.88M)	50μL	100μL
HRP Reagent	100μL	200μL
1x Reaction buffer	10mL	20mL

Storage Conditions: Store the kit at -20℃ protected from light.
Shelf Life: 6 months.

 

Diagram for H2O2 Standard Preparation:

 

Fluorometric Example Standard Curve:                                                      Colorimetric Example Standard Curve:

                                             

Related Products:

1. ATP Activity Assay (TBS2010)
2. ADHP Red Hydrogen Peroxidase Assay Kit (TBS2067)
3. AmplexRed_HRP-System (TBS5026)
4. Tryptase Activity Assay (TBS2101)
5. β-Hexosaminidase Activity Assay (TBS2105)

Additional information

SIZE	200 Reactions, 100 Reactions

Datasheet:

TBS2066_AmplexRed_h2o2_v03_2022

MSDS:

TBS2066_H2O2_MSDS