Fast Hydrogen Peroxide Assay

Fast Hydrogen Peroxide Assay

$120.00$198.00

SKU: TBS2066 Category:
SKUStock SIZE PriceQuantity
TBS2066-100Yes100 reactions $120.00
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TBS2066-200Yes200 reactions $198.00
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Description

Hydrogen Peroxide (H2O2) is a reactive oxygen metabolic byproduct that serves as a key regulator for a number of oxidative stress-related states. It is involved in many pathological processes of diseases such as asthma, inflammatory arthritis, atherosclerosis, diabetic vasculopathy, osteoporosis, neuro-degenerative diseases, Down’s syndrome, and immune system diseases.
Tribioscience’s ADHP-red Hydrogen Peroxide Assay is a sensitive, simple, direct, and HTS-ready colorimetric/fluorometric assay designed to measure H2O2 levels in biological samples. In the presence of Horse Radish Peroxidase (HRP), the ADHP-red probe reacts with H2O2 to produce a resorufin that appears red in color. The resorufin can be measured with absorption 570nm for colorimetric or with a highly stable fluorometric assay with maximum emission of 585nm (Ex/Em = 535/587 nm).

Applications:

  • Detection of HRP activity on liquid systems such as ELISA.
  • Western Blot or other immunoassays.
  • Custom packaging and bulk purchase information is available upon request.

Key Features:

  • Flexible: suitable fo colorimetric and fluorometric methods.
  • Accurate: Use 50μL samples. Detection ranges 0.4-200μM in a 96-well plate for colorimetric and fluorometric assay.
  • Simple and high-throughput: One-step procedure – Just load-incubate-read. Kit can be used for a robust method.
  • Time saving: less than 30-minutes.

Kit Contents:

Component100x Rxns200x Rxns
ADHP Solution60μL120μL
H2O2 Standard (0.88M)50μL100μL
HRP Reagent100μL200μL
1x Reaction buffer10mL20mL

Storage Conditions: Store the kit at -20℃ protected from light.
Shelf Life: 6-months.

Fluorometric Example Standard Curve:
Fluorometric Example Standard Curve

Colorimetric Example Standard Curve:
Colorimetric Example Standard Curve

Additional information

SIZE

200 reactions, 100 reactions

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