Northern Blot Service

Northern Blot Service

Northern blot is a molecular biological technique for gene expression, disease diagnosis, and microRNA detection. The northern analysis first starts from RNA extraction from tissues or cells, which are further separated by denaturing agarose gels. The separated RNAs are transferred to a nylon membrane. RNA on the membrane is then hybridized with complementary probes for detection, which could be labeled with biotin, Digoxigenin conjugated, or radioactive isotope-labeled.

With increasingly strict regulatory requirements on radiation exposure, Tribioscience has specialized in non-radioactive and sensitive Northern Blot development to meet urgent scientific needs. We have established and validated Near Infrared (NIR) Fluorescence Northern Blot technology (Fig. 1).

Our service includes sample preparation, RNA purification, probe synthesis, fluorescence labeling, Glyoxal-based RNA denaturation, detection system optimization, and data analysis.

Main Features of NIR Fluorescent Northern Blot

  • Accurate and reproducible quantification of Northern Blot.
  • Probe directly labeled with near-infrared (IR) dyes.
  • More Sensitive than Chemiluminescent or Digoxigenin conjugated detection.
  • Not need Enzyme/substrate.
  • No radioactive, film, or dark room.
  • The membrane can be easily stripped for re-probe application.
  • Time-saving than other Northern Blot approaches.

Tribioscience aims to save customers time by providing a one-stop service:

  • RNA purification and integration analysis
  • RNA gel separation with a greener denaturing system
  • RNA membrane transfer
  • Sensitive probe labeling
  • Probe hybridization
  • Image development and data analysis

Contact Us:

Email: [email protected]
Phone: +1 (408) 498-0197
Address: 365 San Aleso Ave,
                 Sunnyvale, CA 94085

NIR Fluorescence Northern Blot

 

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