Catalase Assay (TBS2006)

Description

Catalase (EC 1.11.1.6) is an ubiquitous antioxidant which catalyzes the conversion of hydrogen peroxide (H₂O₂) into water (H₂O) and oxygen (O₂). H₂O₂ is an oxidative stress inducer that can cause damage cells at relatively low concentrations. H₂O₂ is also precursor to certain oxidant radical species (e.g. hydroxyl radical). Catalase plays a crucial role to prevent oxidative damage to the cells.

Tribioscience catalase Assay Kit provides a rapid, simple, reproducible, and sensitive tool for catalase activity determination in plasma, serum, urine, and other samples. The catalase activity assay uses enzyme reactions to produce color and fluorescence with the probe. The color intensity of the reaction at 570 nm or fluorescence intensity at λex/em = 530/590 nm is directly proportional to catalase concentration in the sample. The fluorometric assay is more sensitive than colorimetric assay.

APPLICATIONS

Direct Assays: Catalase enzyme activity in serum, plasma, urine, and other bio-samples.

KEY FEATURES

Flexible: Suitable for colorimetric and fluorometric methods.

Accurate: Use 25 µL samples, detection ranges are 1.95-31.25 U/mL in 96-well plate the assay.

Simple and high-throughput: Two-steps procedure: just load-incubate-adding-incubate-read. Kits can be used as a robust method.

KIT CONTENTS

STORAGE AND HANDLING

Store kit at -20°C. Shelf life of 1year. Protect from light.

Shipment with blue ice. Avoid frozen and thaw circles.

TYPICAL DATA

The typical data is provided for only demonstration reference of standard curve shown in Fig.2 for fluorometric assay and Fig.3 for colorimetric assay.

The catalase activity assay as shown in Fig.4

Research use only.

Additional information

Datasheet:

TBS2006_Catalase_Activity_assay_V02_2026

MSDS:

TBS2006-Catalase Assay_MSDS_2024

Research use only.