Horseradish Peroxidase Activity Assay

Description

10-Acetyl-3,7-dihydroxyphenoxazine (ADHP), also called Amplex Red or Ampliflu Red, is a sensitive and stable fluorogenic substrate for horseradish peroxidase (HRP). In the presence of HRP and H2O2, ADHP generates highly fluorescent resorufin in red color that has a maximum absorption of 570 nm and maximum emission of 585 nm. So far ADHP has been known as the most sensitive and stable fluorogenic probe for detecting HRP and H2O2. It has been widely used to detect peroxidase Activity.

Tribioscience’s Peroxidase Activity Fluorometric / Colorimetric Assay provides a quick, efficient, and sensitive method of peroxidase activity.

 

Applications

Detection of HRP activity on the liquid system.

 

Key Features

• Sensitive and accurate: 10 times more sensitive than TMB substrate for HRP detection.
• Simple: Just add- incubate-read style.

Kit Contents

Component	100x Rxns	200x Rxns
ADHP Solution	60 μL	120 μL
Developer	600 μL	1200 μL
HRP standard (1 U/mL)	50 μL	100 μL
1X Assay buffer	10 mL	20 mL

Storage Conditions: Store the kit at -20℃ protected from light.
Shelf Life: 12 months.

 

Diagram for HRP Standard Preparation

 

 

Fluorometric Example Standard Curve:                                                      Colorimetric Example Standard Curve:

                                           

 

Related Products

1. ATP Activity Assay (TBS2010)
2. Amplex Red Hydrogen Peroxide Assay Kit (TBS2066)
3. AmplexRed_HRP-System (TBS5026)
4. Tryptase Activity Assay (TBS2101)
5. β-Hexosaminidase Activity Assay (TBS2105)

Additional information

SIZE	200 Reactions, 100 Reactions

Datasheet:

TBS2067_AmplexRed_HRP assay_v03_2022

MSDS:

TBS2067-AmplexRed_HRP assay_MSDS_2024