MTT Cell Viability AssayTribioscience
MTT assay is one of the most versatile and popular assays. It is based on the cleavage of the yellow tetrazolium salt MTT to purple formazan crystal by metabolic active cells. The formazan is then solubilized, and the concentration determined by optical density at 570 nm. The formazan color intensity is directly proportional to the number of living cells in the culture.
The MTT Cell Viability Assay Kit provides a convenient, sensitive, quantitative, and reliable assay method for the determination of the number of viable cells using standard microplate absorbance readers. Determination of cell growth rates is widely used in the testing of drug action, cytotoxic agents, and screening other biologically active compounds. Reagents in the kit have been carefully formulated and optimized for sensitivity, assay robustness, and automation.