Lactate dehydrogenase (LDH) is a stable cytoplasmic enzyme which is present in most cells. It is released upon damage of the cytoplasmic membrane. The measurement of LDH release is a well-accepted assay to estimate cell membrane integrity and quantify cell cytotoxicity.
The Tribo™ LDH Cytotoxicity Assay Kit uses resazurin as a fluorogenic indicator for measuring the activity of LDH released from damaged cells (Scheme 1). The fluorescent signal is proportional to the number of dead cells. The kit provides an easy and accurate approach to measure LDH activity and cytotoxicity of a variety of compounds.
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