BrdU Cell Proliferation Colorimetric Assay

BrdU Cell Proliferation Colorimetric Assay

$380.00

BrdU Cell Proliferation Colorimetric Assay Kit is designed as a precise, fast and simple colorimetic alternative to quantitate cell proliferation based on the measurement of BrdU incorporation during DNA synthesis in proliferating cells. 5-bromo-2-deoxyuridine (BrdU) is a pyrimidine analog.

SKU: TBS2086 Category:
SKUStock SIZE (test) PriceQuantity
TBS2086Yes200 tests $380.00
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Description

BrdU Cell Proliferation Colorimetric Assay Kit is designed as a precise, fast and simple colorimetic alternative to quantitate cell proliferation based on the measurement of BrdU incorporation during DNA synthesis in proliferating cells. 5-bromo-2-deoxyuridine (BrdU) is a pyrimidine analog. It getsincorporated into the newly synthesized DNA of proliferating cells in place of thymidine. Tribioscience’s BrdU Cell Proliferation Colorimetric Assay Kit detects incorporated BrdU using an anti-BrdU antibody conjugate with Peroxidase (POD) and its substrate. The BrdU antibody POD conjugate allows direct detection of incorporated BrdU without the need for an additional secondary detection antibody. The extent of color development is proportional to the quantity of BrdU incorporated into the cells and can be used directly as an indicator of cell proliferation. Compared to other cell proliferation assays, this kit detects only the proliferating cells and not the seeded cells. This highly sensitive, non-radioactive kit detects as less as 50-100 proliferating cells.

Method:

Blue color staining

Applications:

Detection and quantification of cell proliferation induced by growth factors, cytokines, mitogens, and nutrients. Analysis of cytotoxic and cytostatic compounds such as anticancer drugs, toxic agents and other pharmaceuticals. Determination of the inhibitory or stimulatory effects of various compounds on cell proliferation.

Kit Components and Storage:

Part #NameUnit Size
A1000x Brdu Labeling Reagent30µL
B100x Anti-BrdU-POD300µL
CAntibody Diluent20mL
D10x Washing Buffer25mL
EFixDenat, Ready-to-use45mL
FTMB Substrate25mL
Storage at –20℃.
Shelf-life: 6 month after receipt.

Enough for 200 tests

Additional information

SIZE (test)

200 tests

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