BrdU Cell Proliferation Colorimetric Assay (TBS2086)

$380.00

BrdU Cell Proliferation Colorimetric Assay Kit is designed as a precise, fast and simple colorimetic alternative to quantitate cell proliferation based on the measurement of BrdU incorporation during DNA synthesis in proliferating cells. 5-bromo-2-deoxyuridine (BrdU) is a pyrimidine analog.

SKU: TBS2086 Category:
SKUStock SIZE (test) PriceQuantity
TBS2086 Yes 200 Tests $380.00
spinner

Description

BrdU Cell Proliferation Colorimetric Assay Kit is designed as a precise, fast, and simple colorimetric alternative to quantitate cell proliferation based on the measurement of BrdU incorporation during DNA synthesis in proliferating cells. 5-bromo-2-deoxyuridine (BrdU) is a pyrimidine analog. It gets incorporated into the newly synthesized DNA of proliferating cells in place of thymidine. Tribioscience’s BrdU Cell Proliferation Colorimetric Assay Kit detects incorporated BrdU using an anti-BrdU antibody conjugate with Peroxidase (POD) and its substrate. The BrdU antibody POD conjugate allows direct detection of incorporated BrdU without the need for an additional secondary detection antibody. The extent of color development is proportional to the quantity of BrdU incorporated into the cells and can be used directly as an indicator of cell proliferation. Compared to other cell proliferation assays, this kit detects only the proliferating cells and not the seeded cells. This highly sensitive, non-radioactive kit detects as less as 50-100 proliferating cells.

Method

  • Blue color staining

Applications

Detection and quantification of cell proliferation induced by growth factors, cytokines, mitogens, and nutrients. Analysis of cytotoxic and cytostatic compounds such as anticancer drugs, toxic agents, and other pharmaceuticals. Determination of the inhibitory or stimulatory effects of various compounds on cell proliferation.

Kit Components and Storage

Part # Name Unit Size
A 1000x Brdu Labeling Reagent 30µL
B 100x Anti-BrdU-POD 300µL
C Antibody Diluent 20mL
D 10x Washing Buffer 25mL
E FixDenat, Ready-to-use 45mL
F TMB Substrate 25mL
Storage at –20℃.
Shelf-life: 6 months after receipt.

 

Additional information

SIZE (test)

200 Tests

Reviews

There are no reviews yet.


Be the first to review “BrdU Cell Proliferation Colorimetric Assay (TBS2086)”