Cytochrome C Reductase Activity Assay

Cytochrome C Reductase Activity Assay

$420.00

This kit is designed to measure the NADPHcytochrome c reductase activity in biological samples including cell and tissue extracts, and in purified microsomes (endoplasmic reticulum, ER). Eukaryotic NADPH-Cytochrome c reductase (NADPHcytochrome P450 reductase, EC 1.6.2.4) is a flavoprotein localized to the endoplasmic reticulum.

SKU: TBS2116 Category:
SKUStock SIZE (test) PriceQuantity
TBS2116Yes100 tests $420.00
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Description

This kit is designed to measure the NADPHcytochrome c reductase activity in biological samples including cell and tissue extracts, and in purified microsomes (endoplasmic reticulum, ER).
Eukaryotic NADPH-Cytochrome c reductase (NADPHcytochrome P450 reductase, EC 1.6.2.4) is a flavoprotein localized to the endoplasmic reticulum. It transfers electrons from NADPH to several oxygenases, the most important of which is the cytochrome P450 family of enzymes which are responsible for xenobiotic metabolism. NADPH-cytochrome c reductase is widely used as an endoplasmic reticulum marker and as a biomarker of ecological pollution and dietary lipid uptake.
This kit is based on a colorimetric assay that measures the reduction of cytochrome c by NADPH-Cytochrome c reductase in the presence of NADPH. The reduction of cytochrome c results in the formation of distinct bands in the absorption spectrum and the increase in absorbance at 550 nm is measured with time.

Applications:

Measuring NADPH-cytochrome c reductase levels in biological samples after exposure to drugs or other xenobiotics. Detection of ER during isolation and subcellular fractionation by density gradient separation.

Kit Contents:

Assay Buffer20mL
Enzyme Dilution Buffer2mL
Cytochrome c (100x)0.2mL
NADPH (10x)0.2mL
Cytochrome c Reductase (NADPH)20μL

Enough Reagent for 100 tests

Storage Conditions

Storage Temperature –20℃

Additional information

SIZE (test)

100 tests

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