Annexin V-FITC/PI Apoptosis Kit (TBS2108)

Description

Annexin V-FITC/PI Apoptosis Kit is designed to detect cell apoptosis induced by a variety of stimuli. It is based on the translocation of the membrane phosphatidylserine (PS) from the inner face of the plasma membrane in normal status to the cell surface after apoptosis initiation (Fig.1). PS on the cell surface can be detected by staining with a fluorescent conjugate of Annexin V, a protein that has a high affinity for PS. The detection can be analyzed by flow cytometry or by fluorescence microscopy. The kit can differentiate between apoptosis and necrosis when performing both Annexin V-FITC and propidium iodide (PI) staining. Apoptotic cells show green fluorescence (FITC), dead cells show red (PI) and green fluorescence, and live cells show little or no an argon-ion laser for excitation.

Fig. 1: Annexin V-FITC Apoptosis Detection Principle

Key Features

• Fast: Just 10-15min
• Easy: One step
• Reproducible.

Applications

• Cell apoptosis
• Cell necrosis

Kit Contents for 100 tests:

Storage Conditions

Store the Reagent at 4°C protected from light. Shelf life: 12 months.

Research use only.

Additional information

Datasheet:

TBS2108-Annexin V-FITCPI Apoptosis Kit_Datasheet

MSDS:

TBS2108-Annexin V-FITC-PI Apoptosis Kit_MSDS

Research use only.