Human CD30/TNFRSF18 ELISA (TBS32109)

Description

Tribioscience’s Fast Human CD30 ELISA is designed to quantitatively detect human CD30 levels in different tissues including skin, muscle, neural, serum, and other biological samples. The main feature is that the kit uses our novel proprietary approaches to combine samples and detections into a one-step instead of the complicated traditional methods. It makes the assay simple, easy, accurate and fast. The measurement can be finished in 2 hours, not need 4-5 hours (Fig. 1). The detection range is from 7 to 2000 pg/mL. The levels of human CD30 samples are parallel to the standard curves obtained using the kit standards linearly. These results indicate that this kit can be used to determine relative mass values for natural human CD30 protein.

Typical Data

This standard curve (R2=0.9998) is provided for demonstration only. A standard curve should be generated for each set of samples assayed. Fig. 3 is an example of typical Data.

Sensitivity

The minimum detectable dose (MOD) of human CD30 is typically 10 pg/ml.
The Intra-assay CV is 3.79% the Inter-assay CV is ˂10%.

Specificity

This assay recognizes natural and recombinant human CD30. No cross-reactivity to others.

 

Simple Procedures (Fig. 1)

 

Simple Standard Preparation

 

 

Accurate Standard Curve

 

Kit Content and Storage Conditions

Part	Part#	Description	Storage of Opened/Reconstituted
Human CD30 Microplate	TBS32109A	96 well polystyrene microplate (12 strips of 8 wells) coated with a polyclonal antibody specific for Human CD30.	Return unused wells to the foil pouch. Reseal along the entire edge of the zip-seal. May be stored for up to 1 month at 2-8 °C.
Human CD30 Standard	TBS32109B	20 µL of Recombinant Human CD30 protein (100 ng/mL).	Aliquot and store at -20 ℃ for up to 1 month in a manual defrost freezer. Avoid repeated freeze-thaw cycles.
Detection A	TBS32109C	2.2 mL of Biotin-Human CD30 antibody.	May be stored for up to 3 months at 2-8 °C.
Detection B	TBS32109D	200 µL of Streptavidin-HRP.
Assay Diluent	TBS32109E	25 mL of a buffered protein base with preservatives.
Wash Buffer	TBS3000W	12 mL of concentrated solution (10x).
TMB Substrate	TBS3000T	12 mL of ultra-sensitive TMB substrate.
Stop Solution	TBS3000S	6 mL of 2 N sulfuric acid.

Store the unopened kit at 2-8 ℃. Do not use past kit expiration dates.
The kit contains sufficient materials to run an ELISA on one 96-well plate.

Additional information

SIZE	1 x 96-well plate

Datasheet:

TBS32109_Human CD30_V01 datasheet

MSDS:

TBS32109_Human CD30_ELISA_MSDS