Human GFAP ELISA (TBS32106)
$560.00
SKU | Stock | SIZE | Price | Quantity | ||
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TBS32106 | Yes | 1 x 96-well plate | $560.00 |
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Description
Tribioscience’s Human GFAP ELISA is designed to quantitatively detect human GFAP levels in serum, plasma, and other biological samples. The main feature is that the kit uses our novel proprietary approaches to combine samples and detections into a one-step instead of the complicated traditional methods. It makes the assay simple, easy, accurate, and fast (Fig. 1). The detection range is from 4 to 3000 pg/mL. The levels of human GFAP samples are parallel to the standard curves obtained using the kit standards linearly. Therefore, the kit can be used to determine relative mass values for natural human GFAP protein.
Typical Data
This standard curve (R2=1.000) is provided for demonstration only. A standard curve should be generated for each set of samples assayed. Fig. 3 is an example of typical Data.
Sensitivity
The minimum detectable dose (MOD) of human is typically 4 pg/mL. The Intra-assay CV and the Inter-assay CV are ˂10%.
Specificity
This assay recognizes natural and recombinant human GFAP. No cross-reactivity with others.
Simple Procedures (Fig. 1)
Simple Standard Preparation
Accurate Standard Curve
Kit Content and Storage Conditions
Part | Part# | Description | Storage of Opened/Reconstituted |
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Human GFAP Microplate |
TBS32106A | 96 well polystyrene microplate (12 strips of 8 wells) coated with a monoclonal antibody specific for human GFAP. |
Return unused wells to the foil pouch. Reseal along the entire edge of the zip-seal. May be stored for up to 1 month at 2-8 °C. |
Human GFAP Standard |
TBS32106B | 100 µl of Recombinant human GFAP (30 ng/mL). | Aliquot and store at -20 ℃ for up to 1 month in a manual defrost freezer. Avoid repeated freeze-thaw cycles. |
Detection A | TBS32106C | 2.1 mL of biotin- human GFAP antibody. | May be stored for up to 3 months at 2-8 °C. |
Detection B | TBS32106D | 200 µL of streptavidin HRP. | |
Assay Diluent | TBS32106E | 25 mL of a buffered protein base with preservatives. | |
Wash Buffer | TBS3000W | 12 mL of concentrated solution (10x). | |
TMB Substrate | TBS3000T | 12 mL of ultra-sensitive TMB substrate. | |
Stop Solution | TBS3000S | 6 mL of 2 N sulfuric acid. |
Store the unopened kit at 2-8 ℃. Do not use past kit expiration dates.
The kit contains sufficient materials to run an ELISA on one 96-well plate.
Related Product
Additional information
SIZE | 1 x 96-well plate |
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