Human GFAP ELISA (TBS32106)

Description

Tribioscience’s Human GFAP ELISA is designed to quantitatively detect human GFAP levels in serum, plasma, and other biological samples. The main feature is that the kit uses our novel proprietary approaches to combine samples and detections into a one-step instead of the complicated traditional methods. It makes the assay simple, easy, accurate, and fast (Fig. 1). The detection range is from 4 to 3000 pg/mL. The levels of human GFAP samples are parallel to the standard curves obtained using the kit standards linearly. Therefore, the kit can be used to determine relative mass values for natural human GFAP protein.

 

Typical Data

This standard curve (R²=1.000) is provided for demonstration only. A standard curve should be generated for each set of samples assayed. Fig. 3 is an example of typical Data.

Sensitivity

The minimum detectable dose (MOD) of human is typically 4 pg/mL. The Intra-assay CV and the Inter-assay CV are ˂10%.

Specificity

This assay recognizes natural and recombinant human GFAP. No cross-reactivity with others.

 

Simple Procedures (Fig. 1)

 

Simple Standard Preparation

 

 

Accurate Standard Curve

 

Kit Content and Storage Conditions

Part	Part#	Description	Storage of Opened/Reconstituted
Human GFAP Microplate	TBS32106A	96 well polystyrene microplate (12 strips of 8 wells) coated with a monoclonal antibody specific for human GFAP.	Return unused wells to the foil pouch. Reseal along the entire edge of the zip-seal. May be stored for up to 1 month at 2-8 °C.
Human GFAP Standard	TBS32106B	100 µl of Recombinant human GFAP (30 ng/mL).	Aliquot and store at -20 ℃ for up to 1 month in a manual defrost freezer. Avoid repeated freeze-thaw cycles.
Detection A	TBS32106C	2.1 mL of biotin- human GFAP antibody.	May be stored for up to 3 months at 2-8 °C.
Detection B	TBS32106D	200 µL of streptavidin HRP.
Assay Diluent	TBS32106E	25 mL of a buffered protein base with preservatives.
Wash Buffer	TBS3000W	12 mL of concentrated solution (10x).
TMB Substrate	TBS3000T	12 mL of ultra-sensitive TMB substrate.
Stop Solution	TBS3000S	6 mL of 2 N sulfuric acid.

Store the unopened kit at 2-8 ℃. Do not use past kit expiration dates.
The kit contains sufficient materials to run an ELISA on one 96-well plate.

 

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5. Human AD7 Human AD7C NTP (TBS3297)

Additional information

SIZE	1 x 96-well plate

Datasheet:

TBS32106 human GFAP Elisa datasheet-V02_2023

MSDS:

TBS32106_Human_GFAP_ELISA_MSDS