Human GFAP ELISA (TBS32106)
$560.00
| SKU | Stock | SIZE | Price | Quantity | ||
|---|---|---|---|---|---|---|
| TBS32106 | Yes | 1 x 96-well plate | $560.00 |
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Description
Tribioscience’s Human GFAP ELISA is designed to quantitatively detect human GFAP levels in serum, plasma, and other biological samples. The main feature is that the kit uses our novel proprietary approaches to combine samples and detections into a one-step instead of the complicated traditional methods. It makes the assay simple, easy, accurate, and fast (Fig. 1). The detection range is from 8.23 to 6000 pg/mL. The levels of human GFAP samples are parallel to the standard curves obtained using the kit standards linearly. Therefore, the kit can be used to determine relative mass values for natural human GFAP protein.
Typical Data
This standard curve (R2=1.000) is provided for demonstration only. A standard curve should be generated for each set of samples assayed. Fig. 3 is an example of typical Data.
Sensitivity
The minimum detectable dose (MDD) of human is typically 1.7–2.5 pg/mL.
The Intra-assay CV and the Inter-assay CV are ˂10%.
Specificity
This assay recognizes natural and recombinant human GFAP.
No cross-reactivity with others.
Simple Procedures (Fig. 1)
Simple Standard Preparation
Accurate Standard Curve
Kit Content and Storage Conditions
| PART | PART# | DESCRIPTION | STORAGE OF OPENED/ RECONSTITUTED |
| Human GFAP Microplate | TBS32106A | 96 well polystyrene microplate (12 strips of 8 wells) coated with a monoclonal antibody specific for human GFAP. | Return unused wells to the foil pouch. Reseal along the entire edge of the zip-seal. May be stored for up to 1 month at 2-8 °C. |
| Human GFAP Standard | TBS32106B | 10 µl of Recombinant human GFAP (600 ng/mL). | Aliquot and store at -20 ℃ for up to 1 month in a manual defrost the freezer. Avoid repeated freeze-thaw cycles. |
| Detection A | TBS32106C | 180 µL of biotin-human GFAP antibody (12x). |
May be stored for up to 3 months at 2-8 °C. |
| Detection B | TBS32106D | 330 µL of streptavidin HRP (40x). | |
| Assay Diluent | TBS32106E | 25 mL of a buffered protein base with preservatives. | |
| Wash Buffer | TBS3000W | 12 mL of concentrated solution (10x). | |
| TMB Substrate | TBS3000T | 12 mL of ultra-sensitive TMB substrate. | |
| Stop Solution | TBS3000S | 6 mL of 2 N sulfuric acid. |
Store the unopened kit at 2-8 ℃. Do not use past kit expiration dates.
The kit contains sufficient materials to run an ELISA on one 96-well plate.
Research use only.
Additional information
| SIZE | 1 x 96-well plate |
|---|
Datasheet:
TBS32106 human GFAP Elisa datasheet
MSDS:
TBS32106_Human_GFAP_ELISA_MSDS
Research use only.
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