Fast Human Phospho Tau Thr181 (p-T181) ELISA (TBS3294)

Description

Tribioscience’s Fast Human Phospho Tau Thr181 (p-Tau-181) ELISA is designed to quantitatively detect human p-Tau-181 levels in serum, plasma, CSF, and other biological samples. The main feature is that the kit uses our novel proprietary approaches to combine samples and detections into a one-step instead of the complicated traditional methods. It makes the assay simple, easy, accurate, and fast. The Hands-on time can be within 2 hours, with no need for 4-5 hours (Fig. 1). The detection range is from 0.3 to 243 ng/mL. The levels of human p-Tau-181 samples are parallel to the standard curves obtained using the kit standards linearly. Therefore, the kit can be used to determine relative mass values for natural human p-Tau-181 protein.

 

Typical Data

This standard curve (R²=1.000) is provided for demonstration only. A standard curve should be generated for each set of samples assayed. Fig. 3 is an example of typical Data.

Sensitivity

The minimum detectable dose (MOD) of human is typically 200 pg/ml. The Intra-assay CV and the Inter-assay CV are ˂10%.

Specificity

This assay recognizes natural and recombinant human pTau-181. No cross-reactivity with others.

 

Simple Procedures (Fig. 1)

 

Simple Standard Preparation

Accurate Standard Curve

 

Kit Content and Storage Conditions

Part	Part#	Description	Storage of Opened/Reconstituted
Human p-Tau-181 Microplate	TBS3294A	96 well polystyrene microplate (12 strips of 8 wells) coated with a monoclonal antibody specific for human p-tau181.	Return unused wells to the foil pouch. Reseal along the entire edge of the zip-seal. May be stored for up to 1 month at 2-8 °C.
Human p-Tau-181 Standard	TBS3294B	50 µl of Recombinant human p-tau181 standard (2.43 μg/mL).	Aliquot and store at -20 ℃ for up to 1 month in a manual defrost the freezer. Avoid repeated freeze-thaw cycles.
Detection A	TBS3294C	2.1 ml of human p-Tau-181 biotinylated Detection antibody.	May be stored for up to 3 months at 2-8 °C.
Detection B	TBS3294D	400 µL of Streptavidin-HRP.
Assay Diluent	TBS3294E	25 ml of a buffered protein base with preservatives.
Wash Buffer	TBS3000W	12 mL of concentrated solution (10x).
TMB Substrate	TBS3000T	12 mL of ultra-sensitive TMB substrate.
Stop Solution	TBS3000S	6 mL of 2 N sulfuric acid.

Store the unopened kit at 2-8 ℃. Do not use past kit expiration dates.
The kit contains sufficient materials to run an ELISA on one 96-well plate.

Related Product

1. Fast Human Phospho Tau Thr217 (pT217) ELISA (TBS3293)
2. Fast Human MAPT/Tau (total) ELISA (TBS3295)
3. Fast Human Phospho Tau Thr231 (p-T231) ELISA (TBS3296)
4. Human IL-2 ELISA (TBS3220)
5. Human IL-4 ELISA (TBS3221)

Additional information

SIZE	1 x 96-well plate

Datasheet:

TBS3294_human-Tau181-elisa_datasheet 04062023

MSDS:

TBS3294_Human_ pTau-181_ELISA_MSDS