Tribo™ Human CEA ELISA Kit

Description

The Tribo™ Human Carcinoembryonic Antigen ELISA Kit is designed for in vitro quantitative determination of CarcinoEmbryonic Antigen (CEA) concentrations in human serum and plasma samples. This assay employs an antibody specific for Human Carcino Embryonic Antigen CEA coated on a 96-well plate. Standards and samples are pipetted into the wells and Carcino Embryonic Antigen CEA present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Carcino Embryonic Antigen CEA antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted into the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Carcino Embryonic Antigen CEA bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450nm. The concentration of CEA in the samples is calculated opposite to the standard curve.

Applications:

Direct Assays: quantitative CEA in different tissues.

Key Features:

• Sensitivity: The minimum detectable dose of human CEA is typically less than 2.0 pg/mL.
• Specificity: This assay recognizes recombinant and natural human CEA. No cross-reactivity with any other cytokine is observed.

 

Kit Contents:

Reagents	Quantity
Precoated 96-well Plate (8x 12 strips)	1 plate
Standard	2x 100μL
Detection A	1x 100μL
Detection B	1x 100μL
Simple Diluent (10x Concentrate)	1x 12mL
TMB Substrate Solution	1x 12mL
Stop Solution	1x 12mL
Wash Solution (20x Concentrate)	1x 50mL
Plate Sealers	4

 

Storage Conditions:

Store the kit at 4℃ and standard at -20℃, respectively.
Shelf life: 12 months after receipt.

Additional information

SIZE	1 x 96-well plate

Datasheet:

TBS3210_human-CEA_ELISA

MSDS:

TBS3210_Human_CEA_ELISA_MSDS