Fast Human Phospho Tau Thr217 (pT217) ELISA

Fast Human Phospho Tau Thr217 (pT217) ELISA

$560.00

Cell culture Supernate, Plasma/Serum p-tau-217

SKU: N/A Category:
SKUStock SIZE PriceQuantity
TBS3293Yes1x96-well plate $560.00
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Description

Tribioscience’s Fast Human Phospho Tau Thr217 (p-Tau217) ELISA is designed to quantitatively detect human p-Tau217 levels in serum, plasma, and other biological samples. The main feature is that the kit uses our novel proprietary approaches to combine samples and detections into a one-step instead of the complicated traditional methods. It makes the assay simple, easy, accurate, and fast. The Hands-on time can be within 2 hours, no need for 4-5 hours (Fig. 1). The detection range is from 0.3 to 243 ng/mL. The levels of human p-Tau217 samples are parallel to the standard curves obtained using the kit standards linearly. Therefore, the kit can be used to determine relative mass values for natural human p-Tau217 protein.

 

Typical Data

This standard curve(R2=1.000) is provided for demonstration only. A standard curve should be generated for each set of samples assayed. Fig. 3 is an example of typical Data.

 

Sensitivity

The minimum detectable dose (MOD) of human is typically 200 pg/ml.

The Intra-assay CV and the Inter-assay CV are ˂10%.

 

Specificity

This assay recognizes natural and recombinant human pTau-217.

No cross-reactivity with others.

 

Simple Procedures

TBS3293-3

Simple Standard Preparation

TBS3293-4

Accurate Standard Curve

TBS3293-5

Kit Content and Storage Conditions

PartPart#DescriptionStorage of Opened/Reconstituted
Human PTau-217 MicroplateTBS3293A96 well polystyrene microplate (12 strips of 8 wells) coated with a monoclonal antibody specific for human p-tau217.Return unused wells to the foil pouch. Reseal along the entire edge of the zip-seal. May be stored for up to 1 month at 2-8 °C.
Human PTau-217 StandardTBS3293B50 µl of Recombinant human p-tau217 (2.43 μg/mL).Aliquot and store at -20 ℃ for up to 1 month in a manual defrost the freezer. Avoid repeated freeze-thaw cycles.
Detection ATBS3293C2.1 ml of human p-tau217 Detection antibody.May be stored for up to 3 months at 2-8 °C.
Detection BTBS3293D12 ml of HRP conjugated secondary antibody.
Assay DiluentTBS3293E12 ml of a buffered protein base with preservatives.
Wash BufferTBS3000W12 mL of concentrated solution (10x).
TMB SubstrateTBS3000T12 mL of ultra-sensitive TMB substrate.
Stop SolutionTBS3000S6 mL of 2 N sulfuric acid.

Store the unopened kit at 2-8 ℃. Do not use past kit expiration dates.
The kit contains sufficient materials to run an ELISA on one 96 well plate.

 

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Additional information

SIZE

1×96-well plate

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