Fast Human Phospho Tau Thr217 (pT217) ELISA (TBS3293)

Tribioscience’s Fast Human Phospho Tau Thr217 (p-Tau217) ELISA is designed to quantitatively detect human p-Tau217 levels in serum, plasma, and other biological samples. The main feature is that the kit uses our novel proprietary approaches to combine samples and detections into a one-step instead of the complicated traditional methods. It makes the assay simple, easy, accurate, and fast. The Hands-on time can be within 2 hours, no need for 4-5 hours (Fig. 1). The detection range is from 0.3 to 243 ng/mL. The levels of human p-Tau217 samples are parallel to the standard curves obtained using the kit standards linearly. Therefore, the kit can be used to determine relative mass values for natural human p-Tau217 protein.

 

Typical Data

This standard curve (R²=1.000) is provided for demonstration only. A standard curve should be generated for each set of samples assayed. Fig. 3 is an example of typical Data.

Sensitivity

The minimum detectable dose (MOD) of human is typically 200 pg/ml. The Intra-assay CV and the Inter-assay CV are ˂10%.

Specificity

This assay recognizes natural and recombinant human pTau-217.

No cross-reactivity with others.

 

Simple Procedures

Simple Standard Preparation

Accurate Standard Curve

Kit Content and Storage Conditions

Part	Part#	Description	Storage of Opened/Reconstituted
Human PTau-217 Microplate	TBS3293A	96 well polystyrene microplate (12 strips of 8 wells) coated with a monoclonal antibody specific for human p-tau217.	Return unused wells to the foil pouch. Reseal along the entire edge of the zip-seal. May be stored for up to 1 month at 2-8 °C.
Human PTau-217 Standard	TBS3293B	50 µl of Recombinant human p-tau217 (2.43 μg/mL).	Aliquot and store at -20 ℃ for up to 1 month in a manual defrost the freezer. Avoid repeated freeze-thaw cycles.
Detection A	TBS3293C	2.1 ml of human p-tau217 Detection antibody.	May be stored for up to 3 months at 2-8 °C.
Detection B	TBS3293D	20 µL of HRP conjugated secondary antibody.
Assay Diluent	TBS3293E	25 mL of a buffered protein base with preservatives.
Wash Buffer	TBS3000W	12 mL of concentrated solution (10x).
TMB Substrate	TBS3000T	12 mL of ultra-sensitive TMB substrate.
Stop Solution	TBS3000S	6 mL of 2 N sulfuric acid.

Store the unopened kit at 2-8 ℃. Do not use past kit expiration dates.
The kit contains sufficient materials to run an ELISA on one 96 well plate.