Fast Human Phospho Tau Thr217 (pT217) ELISA
Cell culture Supernate, Plasma/Serum p-tau-217
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Tribioscience’s Fast Human Phospho Tau Thr217 (p-Tau217) ELISA is designed to quantitatively detect human p-Tau217 levels in serum, plasma, and other biological samples. The main feature is that the kit uses our novel proprietary approaches to combine samples and detections into a one-step instead of the complicated traditional methods. It makes the assay simple, easy, accurate, and fast. The Hands-on time can be within 2 hours, no need for 4-5 hours (Fig. 1). The detection range is from 0.3 to 243 ng/mL. The levels of human p-Tau217 samples are parallel to the standard curves obtained using the kit standards linearly. Therefore, the kit can be used to determine relative mass values for natural human p-Tau217 protein.
This standard curve(R2=1.000) is provided for demonstration only. A standard curve should be generated for each set of samples assayed. Fig. 3 is an example of typical Data.
The minimum detectable dose (MOD) of human is typically 200 pg/ml.
The Intra-assay CV and the Inter-assay CV are ˂10%.
This assay recognizes natural and recombinant human pTau-217.
No cross-reactivity with others.
Simple Standard Preparation
Accurate Standard Curve
Kit Content and Storage Conditions
|Part||Part#||Description||Storage of Opened/Reconstituted|
|Human PTau-217 Microplate||TBS3293A||96 well polystyrene microplate (12 strips of 8 wells) coated with a monoclonal antibody specific for human p-tau217.||Return unused wells to the foil pouch. Reseal along the entire edge of the zip-seal. May be stored for up to 1 month at 2-8 °C.|
|Human PTau-217 Standard||TBS3293B||50 µl of Recombinant human p-tau217 (2.43 μg/mL).||Aliquot and store at -20 ℃ for up to 1 month in a manual defrost the freezer. Avoid repeated freeze-thaw cycles.|
|Detection A||TBS3293C||2.1 ml of human p-tau217 Detection antibody.||May be stored for up to 3 months at 2-8 °C.|
|Detection B||TBS3293D||12 ml of HRP conjugated secondary antibody.|
|Assay Diluent||TBS3293E||12 ml of a buffered protein base with preservatives.|
|Wash Buffer||TBS3000W||12 mL of concentrated solution (10x).|
|TMB Substrate||TBS3000T||12 mL of ultra-sensitive TMB substrate.|
|Stop Solution||TBS3000S||6 mL of 2 N sulfuric acid.|
Store the unopened kit at 2-8 ℃. Do not use past kit expiration dates.
The kit contains sufficient materials to run an ELISA on one 96 well plate.