Human MBL Oligomer ELISA Kit (TBS32105)

Description

The Tribio® Human MBL ELISA is a solid phase ELISA designed to measure human MBL levels in serum and plasma. The main feature is that the kit uses our novel proprietary approaches to combine samples and detection into a one-step instead of the complicated traditional methods. It makes the assay simple, easy, accurate, and fast. The measurement can be finished in 2 hours, with no need for 5-6 hours (Fig. 1). The measurement can be finished within 2 hours, not need 4-5 hours. The detection range is from 0.125 to 20 ng/mL. The levels of human MBL samples are parallel to the standard curves obtained using the kit standards linearly. These results indicate that this kit can be used to determine relative mass values for natural human MBL protein levels.

 

Typical Data

This standard curve is provided for demonstration only as Fig.3. A standard curve should be generated for each set of samples assayed.

Sensitivity

The minimum detectable dose (MOD) of human MBL is typically 100 pg/ml.

Specificity

This assay recognizes natural and recombinant human MBL.

 

Simple Procedures (Fig. 1)

 

 

Simple Standard Preparation

 

Accurate Standard Curve

 

Kit Content and Storage Conditions

Part	Part#	Description	Storage of Opened/Reconstituted
HumanMBL Microplate	TBS32105A	96-well strip microplate (12 strips of 8 wells) coated with a polyclonal antibody specific for human MBL.	Return unused wells to the foil pouch. Reseal along the entire edge of the zip-seal. May be stored for up to 1 month at 2-8 °C.
Human MBL Standard (Lyophilized)	TBS32105B	20ng Recombinant human MBL protein. Reconstitute in 0.5mL DD water.	Aliquot and store at -20 ℃ for up to 1 month in a manual defrost freezer. Avoid repeated freeze-thaw cycles.
Detection A	TBS32105C	2.2 ml of Biotin-Human MBL antibody.	May be stored for up to 1 month at 2-8 °C.
Detection B	TBS32105D	300 µL HRP-Streptavidin (1:50 dilution).
Assay Diluent	TBS32105E	12 ml of a buffered protein base with preservatives.
Wash Buffer	TBS3000W	12 mL of concentrated solution (10x).
TMB Substrate	TBS3000T	12 mL of ultra-sensitive TMB substrate.
Stop Solution	TBS3000S	6 mL of 2 N sulfuric acid.

Store the unopened kit at 2-8 ℃. Do not use past kit expiration dates.
The kit contains sufficient materials to run an ELISA on one 96-well plate.

 

Additional information

SIZE	1 x 96-well plate

Datasheet:

TBS32105_human_MBL_Oligomer_elisa_V01_2023

MSDS:

TBS32105_Human_MBL_ELISA_MSDS