Tribo™ Mouse CCL2/MCP-1 ELISA Kit

Tribo™ Mouse CCL2/MCP-1 ELISA Kit

$320.00

The Mouse MCP-1 ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of mouse MCP-1 in different tissue plasma, serum, and other biological samples.

SKU: TBS3081 Category:
SKUStock SIZE PriceQuantity
TBS3081Yes1x96-well plate $320.00
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Description

Monocyte Chemotactic Protein-1 (MCP-1), also known as Chemokine (C-C motif) ligand 2 (CCL2), is a member of the beta (CC) subfamily of chemokines. MCP-1 is characterized as a monocyte-specific chemoattractant. Because of its target cell specificity, MCP-1 is postulated to play a pathogenic role in a variety of diseases characterized by mononuclear cell infiltration, including atherosclerosis, rheumatoid arthritis and allergic responses. In basophils, MCP-1 is highly effective as a stimulus of histamine release but has only weak chemotactic activity. Additionally, it has been shown to chemoattract CD4+ and CD8+ T lymphocytes.
The Mouse MCP-1 ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of mouse MCP-1 in different tissue plasma, serum, and other biological samples. The plate has been coated with murine MCP-1 monoclonal antibody. Any MCP-1 present in the sample or standard will be captured. A biotin-conjugated anti-murine MCP-1 antibody is added followed by streptavidin-horseradish peroxidase (HRP) and an accompanying substrate. The reaction exhibits a blue color in direct proportion to the amount of MCP-1 in the initial sample. The reaction is terminated by the addition of a stop solution, and absorbance is measured at 450 nm. The concentration of MCP-1 in the samples is calculated opposite to the standard curve.
The mouse MCP-1 ELISA is for research use only. Not for diagnostic or therapeutic procedures.

Kit Sizes:

1x 96-well plate

Applications:

Direct Assays: quantitative MCP-1 in plasma and serum.

Key Features:

  • Sensitivity: The minimum detectable dose of mouse MCP-1 is less than 8.0 pg/mL typically.
  • Specificity: This assay recognizes recombinant and natural mouse MCP-1. No cross-reactivity with any of other cytokine is observed.

Kit Contents:

ReagentsQuantity
Precoated 96-well Plate (8x 12 strips)1 Plate
Standard2x 100μL
Detection A1x 100μL
Detection B1x 100μL
Sample Diluent (10x Concentrate)1x 12mL
TMB Substrate Solution1x 12mL
Stop Solution1x 12mL
Wash Solution (20x Concentrate)1x 50mL
Plate Sealers4

Storage Conditions:

Store the kit at 4℃ and standard at -20℃, respectively.
Shelf life: 12 months after receipt.

Additional information

SIZE

1×96-well plate

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