Tribo™ Mouse KC ELISA Kit
Keratinocyte chemoattractant (KC), also known as CXCL1, SCYB1 and N51is a member of the CXC or alpha chemokine family. KC is similar in structure and activity to IL-8 and MIP- 2.
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Keratinocyte chemoattractant (KC), also known as CXCL1, SCYB1, or N51 is a member of the CXC or alpha chemokine family. KC is similar in structure and activity to IL-8 and MIP-2. It is produced by melanocytes, epidermal keratinocytes, monocytes, macrophages, mammary epithelial cells, endothelial cells, neutrophils, fibroblasts and hepatocytes. Expression level of this chemokine is normally very low in most tissues however, it can be significantly elevated in many disease states including rheumatoid arthritis, cancer, and experimental autoimmune encephalopathy, post surgery and other inflammatory responses.
The Tribo™ Mouse KC ELISA Kit is designed to detect mouse KC level quantitatively in different tissues including skin, muscle, neural, serum and other biological samples. The plate has been coated with murine KC monoclonal antibody. Any KC present in the sample or standard will be captured. The procedure calls for a biotin-conjugated anti-murine KC antibody to be added along with streptavidin-horseradish peroxidase (HRP) with an accompanying substrate. The reaction exhibits a blue color in direct proportion to the amount of KC in the initial sample. The reaction is terminated by the addition of a stop solution and absorbance is measured at 450nm. The concentration of KC in the samples is calculated opposite to the standard curve.
Direct Assays: quantitative KC in plasma and serum.
- Sensitivity: The minimum detectable dose of mouse KC is less than 8.0pg/mL typically.
- Specificity: This assay recognizes recombinant and natural mouse KC. No cross-reactivity with any of other cytokine is observed.
1x 96-well plate (8x 12 strip well).
|Precoated 96-well Plate (8x 12 strips)||1 Plate|
|Detection A||1x 100μL|
|Detection B||1x 100μL|
|Sample Diluent (10x Concentrate)||1x 12mL|
|TMB Substrate Solution||1x 12mL|
|Stop Solution||1x 12mL|
|Wash Solution (20x Concentrate)||1x 50mL|
Store the kit at 4℃ and standard at -20℃, respectively.
Shelf life: 12 months after receipt.