Fast Human MAPT/Tau (total) ELISA (TBS3295)

Tribioscience’s Fast Human Tau (Total) ELISA is designed to quantitatively detect human Tau (Total) levels in CSF, serum, plasma, and other biological samples. The main feature is that the kit uses our novel proprietary approaches to combine samples and detections into a one-step instead of the complicated traditional methods. It makes the assay simple, easy, accurate, and fast. The Hands-on time can be within 2 hours, with no need for 4-5 hours (Fig. 1). The detection range is from 8000 to 125 pg/mL. The levels of human Tau (Total) protein in samples are parallel to the standard curves obtained using the kit standards linearly. Therefore, the kit can be used to determine relative mass values for natural human Tau (Total) protein.

Typical Data

This standard curve (R²=1.000) is provided for demonstration only. A standard curve should be generated for each set of samples assayed. Fig. 3 is an example of typical Data.

Sensitivity

The minimum detectable dose (MOD) of human tau total is typically 200 pg/ml.
The Intra-assay CV and the Inter-assay CV are ˂10%.

Specificity

This assay recognizes natural and recombinant human tau total.
No cross-reactivity with others.

Simple Procedures

Simple Standard Preparation

Accurate Standard Curve

Kit Content and Storage Conditions

Part	Part#	Description	Storage of Opened/Reconstituted
Human Tau (Total) Microplate	TBS3295A	96 well polystyrene microplate (12 strips of 8 wells) coated with a monoclonal antibody specific for human Tau (Total).	Return unused wells to the foil pouch. Reseal along the entire edge of the zip-seal. May be stored for up to 1 month at 2-8 °C.
Human Tau (Total) Standard	TBS3295B	30 µl of Recombinant human Tau (Total) (100 ng/mL).	Aliquot and store at -20 ℃ for up to 1 month in a manual defrost the freezer. Avoid repeated freeze-thaw cycles.
Detection A	TBS3295C	2.1 ml of human Tau (Total) Detection antibody.	May be stored for up to 3 months at 2-8 °C.
Detection B	TBS3295D	400 µL of Streptavidin-HRP.
Assay Diluent	TBS3295E	25 mL of a buffered protein base with preservatives.
Wash Buffer	TBS3000W	12 mL of concentrated solution (10x).
TMB Substrate	TBS3000T	12 mL of ultra-sensitive TMB substrate.
Stop Solution	TBS3000S	6 mL of 2 N sulfuric acid.

Store the unopened kit at 2-8 ℃. Do not use past kit expiration dates.
The kit contains sufficient materials to run an ELISA on one 96 well plate.

 

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