Human Gamma-H2AX/rH2AX Fast ELISA (TBS3265)
$760.00
Cell culture Supernate, Plasma/Serum rH2AX
| SKU | Stock | SIZE | Price | Quantity | ||
|---|---|---|---|---|---|---|
| TBS3265 | Yes | 1 x 96-well plate | $760.00 |
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Description
Tribioscience’s Fast Human γ-H2AX ELISA is designed to quantitatively detect human γ-H2AX levels in serum, plasma, and other biological samples. The main feature is that the kit uses our novel proprietary approaches to combine samples and detections into a one-step instead of the complicated traditional methods. It makes the assay simple, easy, accurate, and fast. The Hands-on time can be within 2 hours, with no need for 4-5 hours (Fig. 1). The detection range is from 312.5 to 20000 pg/mL. The levels of human γ-H2AX samples are parallel to the standard curves obtained using the kit standards linearly. Therefore, the kit can be used to determine relative mass values for natural human γ-H2AX protein.
Typical Data
This standard curve (R2=1.000) is provided for demonstration only. A standard curve should be generated for each set of samples assayed. Fig. 3 is an example of typical Data.
Sensitivity
The minimum detectable dose (MOD) of human is typically 10 pg/ml.
The Intra-assay CV and the Inter-assay CV are ˂10%.
Specificity
This assay specifically recognizes Histone H2AX phosphorylated at S139.
No cross-reactivity with unphosphorylated recombinant human (rh) Histone H2AX, rhHistone Macro H2A1, and rhHistone Macro H2A2.
Simple Procedures (Fig.1)
Simple Standard Preparation
Accurate Standard Curve
Kit Content and Storage Conditions
| Part | Part# | Description | Storage of Opened/Reconstituted |
|---|---|---|---|
| Human γ-H2AX Microplate | TBS3265A | 96-well polystyrene microplate (12 strips of 8 wells) coated with a monoclonal antibody specific for human γ-H2AX. | Return unused wells to the foil pouch. Reseal along the entire edge of the zip-seal. May be stored for up to 1 month at 2-8 °C. |
| Human γ-H2AX Standard | TBS3265B | 100 µl of Recombinant human γ-H2AX (200 ng/mL). | Aliquot and store at -20 ℃ for up to 1 month in a manual defrost the freezer. Avoid repeated freeze-thaw cycles. |
| Detection A | TBS3265C | 2.1 ml of biotin- human γ-H2AX antibody. | May be stored for up to 3 months at 2-8 °C. |
| Detection B | TBS3265D | 230 µl of streptavidin HRP (60x). | |
| Assay Diluent | TBS3265E | 25 ml of a buffered protein base with preservatives. | |
| 10x Wash Buffer | TBS3000W | 12 mL of concentrated solution (10x). | |
| TMB Substrate | TBS3000T | 12 mL of ultra-sensitive TMB substrate. | |
| Stop Solution | TBS3000S | 6 mL of 2 N sulfuric acid. |
Store the unopened kit at 2-8 ℃. Do not use past kit expiration dates.
The kit contains sufficient materials to run an ELISA on one 96-well plate.
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Research use only.
Additional information
| SIZE | 1 x 96-well plate |
|---|
Datasheet:
TBS3265_human-rH2AX-elisa_datasheet-V02-2024
MSDS:
TBS3265_Human_γ-H2AX_ELISA_MSDS
Research use only.
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