Human Total-H2AX Fast ELISA

Description

Tribioscience’s Fast Human H2AX ELISA is designed to quantitatively detect human H2AX levels in serum, plasma, and other biological samples. The main feature is that the kit uses our novel proprietary approaches to combine samples and detections into a one-step instead of the complicated traditional methods. It makes the assay simple, easy, accurate, and fast. The Hands-on time can be within 2 hours, and not need 4-5 hours (Fig. 1). The detection range is from 0.3 to 20 ng/mL. The levels of human H2AX samples are parallel to the standard curves obtained using the kit standards linearly. Therefore, the kit can be used to determine relative mass values for natural human H2AX protein.

 

Typical Data

This standard curve (R²=1.000) is provided for demonstration only. A standard curve should be generated for each set of samples assayed. Fig. 3 is an example of typical Data.

Sensitivity

The minimum detectable dose (MOD) of humans is typically 300 pg/ml.
The Intra-assay CV and the Inter-assay CV are ˂10%.

Specificity

This assay recognizes natural and recombinant human H2AX.
No cross-reactivity with others.

 

Simple Procedures (Fig. 1)

Simple Standard Preparation

Accurate Standard Curve

 

Kit Content and Storage Conditions

Part	Part#	Description	Storage of Opened/Reconstituted
Human H2AX Microplate	TBS3266A	96-well polystyrene microplate (12 strips of 8 wells) coated with a monoclonal antibody specific for human H2AX.	Return unused wells to the foil pouch. Reseal along the entire edge of the zip-seal. May be stored for up to 1 month at 2-8 °C.
Human H2AX Standard	TBS3266B	20 µl of Recombinant human H2AX (1 μg/mL).	Aliquot and store at -20 ℃ for up to 1 month in a manual defrost freezer. Avoid repeated freeze-thaw cycles.
Detection A	TBS3266C	2.1 ml of HRP- human H2AX antibody.	May be stored for up to 3 months at 2-8 °C.
Assay Diluent	TBS3266E	12 ml of a buffered protein base with preservatives.
Wash Buffer	TBS3000W	12 mL of concentrated solution (10x).
TMB Substrate	TBS3000T	12 mL of ultra-sensitive TMB substrate.
Stop Solution	TBS3000S	6 mL of 2 N sulfuric acid.

Store the unopened kit at 2-8 ℃. Do not use past kit expiration dates.
The kit contains sufficient materials to run an ELISA on one 96-well plate.

Related Product

1. Fast Human Phospho Tau Thr217 (pT217) ELISA (TBS3293)
2. Fast Human MAPT/Tau (total) ELISA (TBS3295)
3. Fast Human Phospho Tau Thr231 (p-T231) ELISA (TBS3296)
4. Human IL-2 ELISA (TBS3220)
5. Human IL-4 ELISA (TBS3221)

Additional information

SIZE (kit)	1 x 96-Well Plate

Datasheet:

TBS3266_human-H2AX-elisa_datasheet-V01-2022

MSDS:

TBS3266_Human_H2AX_ELISA_MSDS