Equine Insulin Fast ELISA

Equine Insulin Fast ELISA

$410.00

The Equine Insulin ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of equine insulin in serum, plasma, and cell culture supernatants. The microplate is coated with mouse monoclonal antibody specific for Insulin.

SKU: TBS34010 Category:
SKUStock SIZE PriceQuantity
TBS34010Yes96-well plate $410.00
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Description

The Equine Insulin ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of equine insulin in serum, plasma, and cell culture supernatants. The microplate is coated with mouse monoclonal antibody specific for Insulin. Standards or samples are pipetted into the wells, and incubate together with biotinylated anti-insulin monoclonal antibody (Detection A). The insulin present in a sample is bound to the wells by the capture antibody, and then biotinylated antibody, producing an antibody-antigen-antibody “sandwich”. After washing away unbound biotinylated antibody, HRP-streptavidin (Detection B) is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and exhibits a blue color in direct proportion to the amount of insulin in the initial sample. The reaction is terminated by the addition of an acid, and absorbance is measured at 450 nm. The concentration of insulin in the samples is determined by comparing the O.D. of the samples to the standard curve.

Kit Size:

1x 96-well plate

Applications:

Direct Assays: Insulin concentrations in different tissues.

Key Features:

  • Rapid assay: Less than 3 hours.
  • Every reagent is provided in liquid form and ready to use.
  • Excellent precision and reproducibility.
  • A simple assay procedure without any pretreatment of samples.

 

Kit Contents:

ReagentsQuantity
Precoated 96-well Plate1
Standard2x 100 μL
Detection A1x 100 μL
Detection B1x 100 μL
Sample Diluent ( 10 x concentrate)1x 12 ml
TMB Substrate Solution1x 12 ml
Stop Solution1x 12 ml
Wash Solution (20 x concentrate)1x 50 ml
Plate Sealers4

 

Storage Conditions:

Store the kit at 4℃ and standard at -20℃, respectively.
Shelf life: 6 months after receipt.

Additional information

SIZE

96-well plate

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