Tribo™ Mouse G-CSF ELISA Kit (TBS3080)

$320.00

SKU: TBS3080 Category:
SKUStock SIZE PriceQuantity
TBS3080 Yes 1 x 96-well plate $320.00
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Description

The Fast Mouse G-CSF ELISA is a solid phase ELISA designed to measure mouse G-CSF levels in cell culture supernates, serum, and plasma. The main feature is that the kit uses our novel proprietary approaches to combine samples and detections into a one-step instead of the complicated multiple steps in traditional methods. It makes the assay simple, easy, accurate, and fast. The measurement can be finished in 1 hour, not need 4-5 hours (Fig. 1). The detection range is from 8 to 2000 pg/mL. The levels of mouse G-CSF samples are parallel to the standard curves obtained using the kit standards linearly. These results indicate that this kit can be used to determine relative mass values for natural mouse G-CSF protein.

Kit Contents and Storage Conditions

Reagents Quantity
Mouse G-CSF Capture 96 well microplate (12 strips of 8 wells) coated with a capture antibody against mouse G-CSF.
Mouse G-CSF Standard 20 µl of Recombinant mouse G-CSF protein (100ng/mL).
Detection A 2.1 mL of mouse G-CSF antibody-Biotin.
Detection B 240 µl of Streptavidin-HRP (50x).
Assay Diluent 20 ml of a buffered protein base with preservatives.
10x WashBuffer 12ml of concentrated solution (10x).
TMB Substrate 12 ml of ultra-sensitive TMB substrate.
Stop Solution 6ml of 2 N sulfuric acid.

Store the unopened kit at 2-8 ℃. Do not use past kit expiration dates.

 

Fig. 1 Simple Assays Procedures

Fig. 2 Diagram for Mouse G-CSF standard preparation

Fig. 3 Mouse G-CSF Standard Concentration


Additional information

SIZE

1 x 96-well plate

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