Human IFN-Alpha Fast ELISA (TBS3211)

The Tribio® Human IFN-α Fast ELISA is a solid phase ELISA designed to measure human IFN-α levels in cell culture supernatants, serum, and plasma. The main feature is that the kit uses our novel proprietary approaches to combine samples and detection into a one-step instead of the complicated traditional methods. It makes the assay simple, easy, accurate and fast. The measurement can be finished in 2 hours, with no need for 5-6 hours (Fig. 1). The detection range is from 15.6 to 1000 pg/mL. The levels of human IFN-α samples are parallel to the standard curves obtained using the kit standards linearly. These results indicate that this kit can be used to determine relative mass values for natural human IFN-α protein.

 

Typical Data

This standard curve is provided for demonstration only as Fig.3. A standard curve should be generated for each set of samples assayed.

Sensitivity

The minimum detectable dose (MOD) of human IFN-α is typically 8 pg/ml.

Specificity

This assay recognizes natural and recombinant human IFN-α.

 

Simple Procedures (Fig. 1)

 

Simple Standard Preparation

 

Accurate Standard Curve

 

Kit Content and Storage Conditions

Part	Part#	Description	Storage of Opened/Reconstituted
Human IFN-α Microplate	TBS3211A	96 well strip microplate (12 strips of 8 wells) coated with a polyclonal antibody specific for human IFN-α.	Return unused wells to the foil pouch. Reseal along the entire edge of the zip-seal. May be stored for up to 1 month at 2-8 °C.
Human IFN-α Standard	TBS3211B	100 µL of Recombinant human IFN-α protein (145.8ng/mL).	Aliquot and store at -20 ℃ for up to 1 month in a manual defrost freezer. Avoid repeated freeze-thaw cycles.
Detection A	TBS3211C	2.2 ml of HRP-Human IFN-α antibody.	May be stored for up to 3 months at 2-8 °C.
Assay Diluent	TBS3211D	12 ml of a buffered protein base with preservatives.
Wash Buffer	TBS3000W	12 mL of concentrated solution (10x).
TMB Substrate	TBS3000T	12 mL of ultra-sensitive TMB substrate.
Stop Solution	TBS3000S	6 mL of 2 N sulfuric acid.

Store the unopened kit at 2-8 ℃. Do not use past kit expiration dates.
The kit is sufficient for one 96-well plate ELISA.