TBS3245_IL33

Ultra-sensitive Human IL-33 ELISA

DNA Pure Magnetic Beads (DNA Size ≥100bp)
Ultra Sensitive Human VASN / SLITL2 ELISA
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$450.00

This is ultra-sensitive ELISA and can detect the concentration as low as 0.5 pg/mL in cell culture Supernate, Plasma/Serum IL-33.

SKU: TBS3245 Categories: ,
SKUStock SIZE PriceQuantity
TBS3245 Yes 1 x 96-well plate $450.00
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Description

The Tribio® Ultra-sensitive Human IL-33 Fast ELISA is a solid phase ELISA designed to measure human IL-33 levels in cell culture supernatants, serum, and plasma. The main feature is that the kit uses our novel proprietary approaches to combine samples and detection into a one-step instead of the complicated traditional methods. It makes the assay simple, easy, accurate, and ultra-sensitive. The measurement can detect a concentration as low as 0.5pg/mL, and finish in 2 hours, with no need for 5-6 hours (Fig. 1). The detection range is from 0.5 to 2048 pg/mL. The levels of human IL-33 samples are parallel to the standard curves obtained using the kit standards linearly. Therefore, the kit can be used to determine relative mass values for natural human IL-33 protein.

Simple Procedures

 

Simple Standard Preparation

 

Accurate Standard Curve

 

Kit Content and Storage Conditions

Part Part# Description Storage of Opened/Reconstituted
Human IL-33 Microplate TBS3245A 96 well strip microplate (12 strips of 8 wells) coated with a polyclonal antibody specific for human IL-33. Return unused wells to the foil pouch containing the desiccant pack. Reseal along the entire edge of the zip-seal. May be stored for up to 1 month at 2-8 °C.
Human IL-33 Standard TBS3245B 100 µL of Recombinant human IL-33 protein (100 ng/mL). Aliquot and store at -20 ℃ for up to 1 month in a manual defrost freezer. Avoid repeated freeze-thaw cycles.
Detection A TBS3245C 2.2 ml of HRP-Human IL-33 antibody. May be stored for up to 3 months at 2-8 °C.
Assay Diluent TBS3245D 12 ml of a buffered protein base with preservatives.
Wash Buffer TBS3000W 12 mL of concentrated solution (10x).
TMB Substrate TBS3000T 12 mL of ultra-sensitive TMB substrate.
Stop Solution TBS3000S 6 mL of 2 N sulfuric acid.

Store the unopened kit at 2-8 ℃. Do not use past kit expiration dates.
The kit contains sufficient materials to run an ELISA on one 96-well plate.

 

Related Products

  1. Human IL-1ß ELISA (TBS3219)
  2. Human IL-2 ELISA (TBS3220)
  3. Human IL-4 ELISA (TBS3221)
  4. Human IL-6 ELISA (TBS3223)
  5. Human IL-7 ELISA (TBS3224)

 

Research use only.

Additional information

SIZE

1 x 96-well plate

Datasheet:

TBS3245_human_IL33_elisa_V02_2021

MSDS:

TBS3245_Human IL-33_ELISA_MSDS

Research use only.

1. Tan, M., Ye, W., Liu, Y., Chen, X., Huttad, L., Chua, M. S., & So, S. (2024). Niclosamide Prodrug Enhances Oral Bioavailability and Targets Vasorin-TGFβ Signaling in Hepatocellular Carcinoma. biorxiv. https://doi.org/10.1101/2024.10.15.618538

2. Tan, M., Ye, W., Liu, Y., Chen, X., Huttad, L., Chua, M. S., & So, S. (2025). Niclosamide Prodrug Enhances Oral Bioavailability and Efficacy Against Hepatocellular Carcinoma by Targeting Vasorin-TGFβ Signaling. Authorea. https://doi.org/10.22541/au.174119415.55528730/v1

Research use only.

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