Human IL-2 ELISA; 1 step and 1hr
The Fast Human IL-2 ELISA is a solid phase ELISA designed to measure human IL-2 levels in cell culture supernatants, serum, and plasma.
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Cell culture Supernate, Plasma/Serum IL-2
Interleukin-2 (T-cell growth factor, TCGF, Aldesleukin, and IL2), is a secreted protein that belongs to the IL-2 family. IL-2 is produced by T-cells in response to antigenic or mitogenic stimulation, which is required for T-cell proliferation and other activities crucial to regulation of the immune response. A recombinant form of IL-2 for clinical use has been approved by the Food and Drug Administration (FDA) for the treatment of cancers (malignant melanoma, renal cell cancer), and is in clinical trials for the treatment of chronic viral infections, and as a booster (adjuvant) for vaccines.
The Fast Human IL-2 ELISA is a solid-phase ELISA designed to measure human IL-2 levels in cell culture supernatants, serum, and plasma. The main feature is that the kit uses our novel proprietary approaches to combine samples and detections into a one-step instead of the complicated multiple steps in traditional methods. It makes the assay simple, easy, accurate, and fast. The measurement can be finished in 1 hour, not need 4-5 hours (Fig. 1). The detection arrangement is from 8 to 2000pg/mL. The levels of human IL-2 samples are parallel to the standard curves obtained using the kit standards linearly. These results indicate that this kit can be used to determine relative mass values for natural human IL-2 protein.
- Novel Proprietary ELISA Approach
- Fast: Only 1 hour, not 4-5 hrs.
- Simple: Sample and Detection combined into one step.
- Easy: All procedures are performed at room temperature.
- High Sensitivity: The lowest detection limit is 8.0 pg/ml.
Principle of the Assay
Kit Content and Storage Condition
This standard curve(R2=0.9991) is provided for demonstration only. A standard curve should be generated for each set of samples assayed. Fig. 3 is an example of typical data.
The minimum detectable dose (MOD) of human IL-2 is typically 10 pg/ml.
The Intra-assay CV is 5.45% the Inter-assay CV is 8.6%.
This assay recognizes natural and recombinant human IL-2.
No cross reaction: Human IL-2 Rα, Human IL-2 Rβ, Human IL-2 Rγ, Mouse IL-2
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