Human IL-4 ELISA; 1 step and 1hr

Description

Interleukin-4 (IL4), is a secreted protein that belongs to the IL-4 / IL-13 family. IL-4 enhances both secretion and cell surface expression of IgE and IgG1. It regulates the expression of the low-affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. IL-4 is essential for the switching of B cells to IgE antibody production and for the maturation of T helper (Th) cells toward the Th2 phenotype. It participates in at least several B-cell activation processes.

The Fast Human IL-4 ELISA is a solid phase ELISA designed to measure human IL-4 levels in cell culture supernatants, serum, and plasma.

 

Key Features

• Novel Proprietary ELISA Approach
• Fast: Only 1 hour,  not 5-6 hrs.
• Simple: Sample and Detection combined into one step.
• Easy: All procedures are performed at room temperature.
• High Sensitivity: The low detection limit is 8.0pg/mL.

The levels of human IL-4 samples are parallel to the standard curves obtained using the kit standards linearly. These results indicate that this kit can be used to determine relative mass values for natural human IL-4 protein.

 

Typical Data

This standard curve (R²=0.999) is provided for demonstration only. A standard curve should be generated for each set of samples assayed as Fig. 3.

Sensitivity

The minimum detectable dose (MOD) of human IL-4 is typically 8.0 pg/ml. The Intra-CV is 4.8% the Inter-CV is 8.6%.

Specificity

This assay recognizes natural and recombinant human IL-4. No cross reactivity: Human IL-4 Rα, Mouse IL-4.

 

 

Simple Procedures

 

Simple Standard Preparation

 

Accurate Standard Curve

 

Kit Content and Storage Conditions

Part	Part#	Description	Storage of Opened/Reconstituted
Human IL-4 Microplate	TBS3232A	96-well microplate (12 strips of 8 wells) coated with a Capture Antibody specific for human IL-4.	The unused wells can be stored in the sealed foil pouch containing the desiccant pack for up to 1 month at 2-8 °C.
Human IL-4 Standard	TBS3232B	96-well microplate (12 strips of 8 wells) coated with a Capture Antibody specific for human IL-4.	Aliquot and store at -20 ℃ for up to 1 month in a manual defrost freezer. Avoid repeated freeze-thaw cycles.
Detection A	TBS3232C	2.2 ml of human IL-4 antibody.	May be stored for up to 3 months at 2-8 °C.
Detection B	TBS3232D	2.2 ml of Streptavidin-HRP.
Assay Diluent	TBS3232E	12 mL of a buffered protein base with preservatives.
10x Wash Buffer	TBS3000W	12 mL of concentrated solution (10x).
TMB Substrate	TBS3000T	12 mL of ultra-sensitive TMB substrate.
Stop Solution	TBS3000S	6 mL of 2 N sulfuric acid.

Store the unopened kit at 2-8 ℃. Do not use past kit expiration dates.
The kit contains sufficient materials to run an ELISA on one 96-well plate.

 

Related Product

1. Human IL-1ß ELISA (TBS3219)
2. Human IL-2 ELISA (TBS3220)
3. Human IL-6 ELISA (TBS3223)
4. Human IL-7 ELISA (TBS3224)
5. Human IL-8 ELISA (TBS3225)

Additional information

SIZE	1 x 96-well plate

Datasheet:

TBS3221_human_IL4_elisa_08_2020

MSDS:

TBS3221_human_IL4_ELISA_MSDS