Ultra Sensitive Human VASN/SLITL2 ELISA

Ultra Sensitive Human VASN/SLITL2 ELISA

$450.00

This ELISA can detect the low concentration of VASN at 1.0pg/mL in cell culture Supernate, Plasma/Serum VASN.

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SKUStock SIZE PriceQuantity
TBS3246Yes1x96-well plate $450.00
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Description

Vasorin (VASN) is a typical type I membrane protein. It contains 1 EGF-like domain, 1 fibronectin type-III domain, 10 LRR (leucine-rich) repeats, 1 LRRCT domain, and 1 LRRNT domain. Vasorin is predominantly expressed in vascular smooth muscle cells, and that its expression is developmentally regulated. It directly binds to transforming growth factor (TGF)-β and attenuates TGF-β signaling in vitro. This suggests that down-regulation of vasorin expression contributes to neointimal formation after vascular injury and that vasorin modulates cellular responses to pathological stimuli in the vessel wall.

The Tribio® Ultra-sensitive Human VASN Fast ELISA is a solid phase ELISA designed to measure human VASN levels in cell culture supernatants, serum, and plasma. The main feature is that the kit uses our novel proprietary approaches to combine samples and detection into a one-step instead of the complicated traditional methods. It makes the assay simple, easy, accurate, and ultra-sensitive. The measurement can detect the concentration as low as 1.0 pg/mL, and finished in 2 hours, not need 5-6 hours (Fig. 1). The detection arrangement is from 1.0 to 2048 pg/mL. The levels of human VASN samples are parallel to the standard curves obtained using the kit standards linearly. Therefore, the kit can be used to determine relative mass values for natural human VASN protein.

 

PRINCIPLE OF THE ASSAY

This assay employs the quantitative e sandwich enzyme immunoassay technique. A monoclonal antibody specific for human VASN was pre-coated onto a microplate. Standards and samples are pipetted into the wells, and then, incubated with an HRP-conjugated detection antibody specific for human VASN. Following a wash to remove any unbound antibodies and samples, an ultra-sensitive TMB substrate solution is added to the wells for color development. The color intensity is in proportion to the amount of VASN bound in the initial step. The intensity of the color is measured by plate read at 450 nm.

 

KIT CONTENT AND STORAGE CONDITIONS

PartPart#DescriptionStorage of Opened/Reconstituted
Human VASN MicroplateTBS3246A96 well strip microplate (12 strips of 8 wells) coated with a polyclonal antibody specific for human VASN.Return unused wells to the foil pouch containing the desiccant pack. Reseal along the entire edge of the zip-seal.  May be stored for up to 1 month at 2-8 °C.
Human VASN StandardTBS3246B100 µL of Recombinant human VASN protein (20480 pg/mL).Aliquot and store at -20 ℃ for up to 1 month in a manual defrost the freezer. Avoid repeated freeze-thaw cycles.
Detection ATBS3246C2.2 ml of HRP-Human VASN antibody.May be stored for up to 3 months at 2-8 °C.
Assay DiluentTBS3246D12 ml of a buffered protein base with preservatives.
Wash BufferTBS3000W12 mL of concentrated solution (10x).
TMB SubstrateTBS3000T12 mL of ultra-sensitive TMB substrate.
Stop SolutionTBS3000S6 mL of 2 N sulfuric acid.

Store the unopened kit at 2-8 ℃. Do not use past kit expiration dates.
The kit contains sufficient materials to run an ELISA on one 96 well plate.

 

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  4. Human IL-6 ELISA (TBS3223)
  5. Human IL-7 ELISA (TBS3224)

 

Additional information

SIZE

1×96-well plate

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