- Additional information
- Reviews (0)
Vasorin (VASN) is a typical type I membrane protein. It contains 1 EGF-like domain, 1 fibronectin type-III domain, 10 LRR (leucine-rich) repeats, 1 LRRCT domain, and 1 LRRNT domain. Vasorin is predominantly expressed in vascular smooth muscle cells, and that its expression is developmentally regulated. It directly binds to transforming growth factor (TGF)-β and attenuates TGF-β signaling in vitro. This suggests that down-regulation of vasorin expression contributes to neointimal formation after vascular injury and that vasorin modulates cellular responses to pathological stimuli in the vessel wall.
The Tribio® Ultra-sensitive Human VASN Fast ELISA is a solid phase ELISA designed to measure human VASN levels in cell culture supernatants, serum, and plasma. The main feature is that the kit uses our novel proprietary approaches to combine samples and detection into a one-step instead of the complicated traditional methods. It makes the assay simple, easy, accurate, and ultra-sensitive. The measurement can detect the concentration as low as 1.0 pg/mL, and finished in 2 hours, not need 5-6 hours (Fig. 1). The detection arrangement is from 1.0 to 2048 pg/mL. The levels of human VASN samples are parallel to the standard curves obtained using the kit standards linearly. Therefore, the kit can be used to determine relative mass values for natural human VASN protein.
PRINCIPLE OF THE ASSAY
This assay employs the quantitative e sandwich enzyme immunoassay technique. A monoclonal antibody specific for human VASN was pre-coated onto a microplate. Standards and samples are pipetted into the wells, and then, incubated with an HRP-conjugated detection antibody specific for human VASN. Following a wash to remove any unbound antibodies and samples, an ultra-sensitive TMB substrate solution is added to the wells for color development. The color intensity is in proportion to the amount of VASN bound in the initial step. The intensity of the color is measured by plate read at 450 nm.
KIT CONTENT AND STORAGE CONDITIONS
|Part||Part#||Description||Storage of Opened/Reconstituted|
|Human VASN Microplate||TBS3246A||96 well strip microplate (12 strips of 8 wells) coated with a polyclonal antibody specific for human VASN.||Return unused wells to the foil pouch containing the desiccant pack. Reseal along the entire edge of the zip-seal. May be stored for up to 1 month at 2-8 °C.|
|Human VASN Standard||TBS3246B||100 µL of Recombinant human VASN protein (20480 pg/mL).||Aliquot and store at -20 ℃ for up to 1 month in a manual defrost the freezer. Avoid repeated freeze-thaw cycles.|
|Detection A||TBS3246C||2.2 ml of HRP-Human VASN antibody.||May be stored for up to 3 months at 2-8 °C.|
|Assay Diluent||TBS3246D||12 ml of a buffered protein base with preservatives.|
|Wash Buffer||TBS3000W||12 mL of concentrated solution (10x).|
|TMB Substrate||TBS3000T||12 mL of ultra-sensitive TMB substrate.|
|Stop Solution||TBS3000S||6 mL of 2 N sulfuric acid.|
Store the unopened kit at 2-8 ℃. Do not use past kit expiration dates.
The kit contains sufficient materials to run an ELISA on one 96 well plate.
- Human IL-1ß ELISA (3219)
- Human IL-2 ELISA (TBS3220)
- Human IL-4 ELISA (TBS3221)
- Human IL-6 ELISA (TBS3223)
- Human IL-7 ELISA (TBS3224)