Tribo™ Mouse IL-2 ELISA Kit

Tribo™ Mouse IL-2 ELISA Kit

$320.00

Interleukin-2 (T-cell growth factor, TCGF, Aldesleukin, and IL2), is a secreted protein that belongs to the IL-2 family. IL-2 is produced by T-cells in response to antigenic or mitogenic stimulation, which is required for T-cell proliferation and other activities crucial to regulation of the immune response. IL-2 displays significant anti-tumor activity for a variety of tumor cell types. A recombinant form of IL-2 for clinical use has been approved by the Food and Drug Administration (FDA) for the treatment of cancers (malignant melanoma, renal cell cancer), and is in clinical trials for the treatment of chronic viral infections, and as a booster (adjuvant) for vaccines.

SKU: TBS3031 Category:
SKUStock SIZE PriceQuantity
TBS3031Yes1x96-well plate $320.00
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Description

Tribo™ Mouse IL-2 ELISA Kit

Interleukin-2 (T-cell growth factor, TCGF, Aldesleukin, and IL2), is a secreted protein that belongs to the IL-2 family. IL-2 is produced by T-cells in response to antigenic or mitogenic stimulation, which is required for T-cell proliferation and other activities crucial to regulation of the immune response. IL-2 displays significant anti-tumor activity for a variety of tumor cell types. A recombinant form of IL-2 for clinical use has been approved by the Food and Drug Administration (FDA) for the treatment of cancers (malignant melanoma, renal cell cancer), and is in clinical trials for the treatment of chronic viral infections, and as a booster (adjuvant) for vaccines.

The Fast Mouse IL-2 ELISA is a solid phase ELISA designed to measure mouse IL-2 levels in cell culture supernatants, serum, and plasma. The main feature is that the kit uses our novel proprietary approaches to combine samples and detections into a one-step instead of the complicated multiple steps in traditional methods. It makes the assay simple, easy, accurate, and fast. The measurement can be finished in 2 hours, not need 4-5 hours (Fig. 1). The detection arrangement is from 8 to 2000pg/mL. The levels of mouse IL-2 samples are parallel to the standard curves obtained using the kit standards linearly. These results indicate that this kit can be used to determine relative mass values for natural mouse IL-2 protein.

Kit Sizes:

1x 96-well plate ( 8×12 strip well).

Key Features:

  • Sensitivity: The minimum detectable dose of mouse IL2 is
    typically less than 3.0 pg/mL.
  • Specificity: This assay recognizes recombinant and natural
    mouse IL2.

Applications:

Direct Assays: Mouse IL2 concentrations in different tissues.

 

Kit Contents:

ReagentsQuantity
Mouse IL-2 Microplate96 well microplate (12 strips of 8 wells) coated with a
Capture Antibody specific for mouse IL-2.
Mouse IL-2 Standard30 µl of Recombinant mouse IL-2 protein (100ng/mL).
Detection A2.2 ml of mouse IL-2 antibody.
Detection B100 µl of Streptavidin-HRP (100x)
Assay Diluent15 ml of a buffered protein base with preservatives.
10x WashBuffer12ml of concentrated solution(10x).
TMB Substrate12 ml of ultra-sensitive TMB substrate.
Stop Solution 6ml of 2 N sulfuric acid.

 

Storage Conditions:

  • Store the kit at 4℃ and standard at -20℃, respectively.
  • Shelf life: 3 months after receipt.

 

Fig. 1 Simple Assays Procedures

 

Fig. 2 Diagram for Mouse IL-2 standard preparation

 

Fig.3 Mouse IL2 standard curve

 

Related Products:

  1. Tribo™ Mouse IL-1β ELISA Kit (TBS3030)
  2. Tribo™ Mouse IL-4 ELISA Kit (TBS3032)
  3. Tribo™ Mouse IL-6 ELISA Kit (TBS3040)
  4. Tribo™ Mouse IL-10 ELISA Kit (TBS3044)
  5. Tribo™ Mouse IL-12 p70 ELISA Kit (TBS3047)

 

Additional information

SIZE

1×96-well plate

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