Tribo™ Mouse IL-2 ELISA Kit (TBS3031)

Description

The Fast Mouse IL-2 ELISA is a solid phase ELISA designed to measure mouse IL-2 levels in cell culture supernatants, serum, and plasma. The main feature is that the kit uses our novel proprietary approaches to combine samples and detections into a one-step instead of the complicated multiple steps in traditional methods. It makes the assay simple, easy, accurate, and fast. The measurement can be finished in 2 hours, not need 4-5 hours (Fig. 1). The detection range is from 8 to 2000 pg/mL. The levels of mouse IL-2 samples are parallel to the standard curves obtained using the kit standards linearly. These results indicate that this kit can be used to determine relative mass values for natural mouse IL-2 protein.

 

Key Features

• Sensitivity: The minimum detectable dose of mouse IL2 is typically less than 3.0 pg/mL.
• Specificity: This assay recognizes recombinant and natural mouse IL2.

 

Applications

Direct Assays: Mouse IL2 concentrations in different tissues.

 

Kit Contents

Reagents	Quantity
Mouse IL-2 Microplate	96 well microplate (12 strips of 8 wells) coated with a Capture Antibody specific for mouse IL-2.
Mouse IL-2 Standard	30 µl of Recombinant mouse IL-2 protein (100ng/mL).
Detection A	2.2 ml of mouse IL-2 antibody.
Detection B	100 µl of Streptavidin-HRP (100x)
Assay Diluent	15 ml of a buffered protein base with preservatives.
10x WashBuffer	12ml of concentrated solution(10x).
TMB Substrate	12 ml of ultra-sensitive TMB substrate.
Stop Solution	6ml of 2 N sulfuric acid.

 

Storage Conditions

• Store the kit at 4℃ and standard at -20℃, respectively.
• Shelf life: 3 months after receipt.

 

Fig. 1 Simple Assays Procedures

 

Fig. 2 Diagram for Mouse IL-2 standard preparation

 

Fig.3 Mouse IL2 standard curve

Additional information

SIZE	1 x 96-well plate

Datasheet:

TBS3031_Mouse_IL2_elisa_v03-03_2021

MSDS:

TBS3031_Mouse_IL2_ELISA_MSDS