Fast Human Granzyme B ELISA; 1 step and 1hr.
$460.00
Cell culture Supernate, Plasma/Serum Granzyme B
SKU | Stock | SIZE | Price | Quantity | ||
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TBS3208 | Yes | 1 x 96-well plate | $460.00 |
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Description
Tribioscience’s Fast Human Granzyme B ELISA is designed to quantitatively detect human Granzyme B levels in serum, plasma, and other biological samples. It is a novel ELISA, different from the traditional ELISA approach. The kit uses our novel proprietary approaches to combine samples and detections into a one-step instead of the complicated traditional methods. It makes the assay simple, easy, accurate, and fast. The measurement can be finished in less than 2 hours, with no need for 4-5 hours (Fig. 1). The detection range is from 8 to 2000 pg/mL. The levels of human Granzyme B samples are parallel to the standard curves obtained using the kit standards linearly. Therefore, the kit can be used to determine relative mass values for natural human Granzyme B protein.
Typical Data
This standard curve(R2=0.996) is provided for demonstration only. A standard curve should be generated for each set of samples assayed. Fig. 3 is an example of typical Data.
Sensitivity
The minimum detectable dose (MOD) of human is typically 10 pg/ml.
The Intra-assay CV and the Inter-assay CV are ˂10%.
Specificity
This assay recognizes natural and recombinant human Granzyme B.
No cross-reactivity with others.
Simple Procedures
Simple Standard Preparation
Accurate Standard Curve
Kit Content and Storage Conditions
Part | Part# | Description | Storage of Opened/Reconstituted |
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Human Granzyme B Microplate | TBS3208A | 96 well polystyrene microplate (12 strips of 8 wells) coated with a monoclonal antibody specific for human Granzyme. | Return unused wells to the foil pouch. Reseal along the entire edge of the zip-seal. May be stored for up to 1 month at 2-8 °C. |
Human Granzyme B Standard | TBS3208B | 30 µl of Recombinant human Granzyme B (100ng/mL). | Aliquot and store at -20 ℃ for up to 1 month in a manual defrost the freezer. Avoid repeated freeze-thaw cycles. |
Detection A | TBS3208C | 2.1 ml of Biotin-human Granzyme B antibody. | May be stored for up to 3 months at 2-8 °C. |
Detection B | TBS3208D | 12 ml of Streptavidin-HRP. | |
Assay Diluent | TBS3206E | 12 ml of a buffered protein base with preservatives. | |
Wash Buffer | TBS3000W | 12 mL of concentrated solution (10x). | |
TMB Substrate | TBS3000T | 12 mL of ultra-sensitive TMB substrate. | |
Stop Solution | TBS3000S | 6 mL of 2 N sulfuric acid. |
Store the unopened kit at 2-8 ℃. Do not use past kit expiration dates.
The kit contains sufficient materials to run an ELISA on one 96 well plate.
Related Product
Additional information
SIZE | 1 x 96-well plate |
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