Tribo™ Mouse IL-6 ELISA Kit (TBS3040)
$320.00 – $560.00
Interleukin-6 (IL-6) is a multifunctional proinflammatory cytokine which regulates immune response, acute-phase reactions, and hematopoiesis. IL-6 is produced by a variety of cells such as hepatocytes, B and T lymphocytes, neurons, tumor cells, and multi-potent hematopoietic cells that acts on a wide range of tissues.
| SKU | Stock | SIZE | Price | Quantity | ||
|---|---|---|---|---|---|---|
| TBS3040-01 | Yes | 1 x 96-well plate | $320.00 | |||
| TBS3040-02 | Yes | 2 x 96-well plate | $560.00 |
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Description
Interleukin-6 (IL-6) is a multifunctional proinflammatory cytokine that regulates immune response, acute-phase reactions, and hematopoiesis. IL-6 is produced by a variety of cells such as hepatocytes, B and T lymphocytes, neurons, tumor cells, and multi-potent hematopoietic cells that act on a wide range of tissues. It plays a central role in the production of a variety of pathological conditions including inflammatory responses, sepsis, rheumatoid arthritis, allergy, trauma, pain, neurodegenerative diseases, as well as several other autoimmune diseases.
The Tribo™ Mouse IL-6 ELISA Kit is designed to detect mouse IL-6 levels quantitatively in different tissues including skin, muscle, neural, serum, and other biological samples. The plate has been coated with murine IL-6 monoclonal antibody and any IL-6 present in the sample or standard will be captured. A biotin-conjugated anti-murine IL-6 antibody is added along with streptavidin-horseradish peroxidase (HRP) accompanied by substrate. The reaction exhibits a blue color in direct proportion to the amount of IL-6 in the initial sample. The reaction can be terminated by the addition of a stop solution and absorbance is measured at 450nm. The concentration of IL-6 in the samples is calculated opposite to the standard curve.
Applications
Direct Assays: quantitative IL-6 in different tissues.
Key Features
- Sensitivity: The minimum detectable dose of mouse IL-6 is less than 8.0 pg/mL typically.
- Specificity: This assay recognizes recombinant and natural mouse IL-6. No cross-reactivity with any of the other cytokine is observed.
Kit Contents
| Reagents | Quantity |
|---|---|
| Precoated 96-well Plate | 1 |
| Standard | 2x 100 μL |
| Detection A | 1x 100 μL |
| Detection B | 1x 100 μL |
| Sample Diluent (10x concentrate) | 1x 12 ml |
| TMB Substrate Solution | 1x 12 ml |
| Stop Solution | 1x 12 ml |
| Wash Solution (20x concentrate) | 1x 50 ml |
| Plate Sealers | 4 |
Storage Conditions
Store the kit at 4℃ and standard at -20℃, respectively.
Shelf life: 12 months after receiving.
Additional information
| SIZE | 1 x 96-well plate, 2 x 96-well plate |
|---|
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