Tribo™ Mouse IL-6 ELISA Kit

Tribo™ Mouse IL-6 ELISA Kit

$320.00$560.00

Interleukin-6 (IL-6) is a multifunctional proinflammatory cytokine which regulates immune response, acute-phase reactions, and hematopoiesis. IL-6 is produced by a variety of cells such as hepatocytes, B and T lymphocytes, neurons, tumor cells, and multi-potent hematopoietic cells that acts on a wide range of tissues.

SKU: TBS3040 Category:
SKUStock SIZE PriceQuantity
TBS3040-01Yes1x96-well plate $320.00
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TBS3040-02Yes2x96-well plate $560.00
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Description

Interleukin-6 (IL-6) is a multifunctional proinflammatory cytokine which regulates immune response, acute-phase reactions, and hematopoiesis. IL-6 is produced by a variety of cells such as hepatocytes, B and T lymphocytes, neurons, tumor cells, and multi-potent hematopoietic cells that acts on a wide range of tissues. It plays a central role in the production of a variety of pathological conditions including inflammatory responses, sepsis, rheumatoid arthritis, allergy, trauma, pain, neurodegenerative diseases, as well as several other autoimmune diseases.
The Tribo™ Mouse IL-6 ELISA Kit is designed to detect mouse IL-6 levels quantitatively in different tissue including skin, muscle, neural, serum, and other biological samples. The plate has been coated with murine IL-6 monoclonal antibody and any IL-6 present in the sample or standard will be captured. A biotin-conjugated anti-murine IL-6 antibody is added along with streptavidin-horseradish peroxidase (HRP) accompanied by substrate. The reaction exhibits a blue color in direct proportion to the amount of IL-6 in the initial sample. The reaction can be terminated by the addition of a stop solution and absorbance is measured at 450nm. The concentration of IL-6 in the samples is calculated opposite to the standard curve.

Kit Sizes:

1x 96-well plate (8x 12 strip well).

Applications:

Direct Assays: quantitative IL-6 in different tissues.

Key Features:

  • Sensitivity: The minimum detectable dose of mouse IL-6 is less than 8.0 pg/mL typically.
  • Specificity: This assay recognizes recombinant and natural mouse IL-6. No cross-reactivity with any of other cytokine is observed.

Kit Contents:

ReagentsQuantity
Precoated 96-well Plate1
Standard2x 100 μL
Detection A1x 100 μL
Detection B1x 100 μL
Sample Diluent (10x concentrate)1x 12 ml
TMB Substrate Solution1x 12 ml
Stop Solution1x 12 ml
Wash Solution (20x concentrate)1x 50 ml
Plate Sealers4

Storage Conditions:

Store the kit at 4℃ and standard at -20℃, respectively.
Shelf life: 12 months after receiving.

Additional information

SIZE

1×96-well plate, 2×96-well plate

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