Tribo™ Mouse IL-6 ELISA Kit
$320.00 – $560.00
Interleukin-6 (IL-6) is a multifunctional proinflammatory cytokine which regulates immune response, acute-phase reactions, and hematopoiesis. IL-6 is produced by a variety of cells such as hepatocytes, B and T lymphocytes, neurons, tumor cells, and multi-potent hematopoietic cells that acts on a wide range of tissues.
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Interleukin-6 (IL-6) is a multifunctional proinflammatory cytokine which regulates immune response, acute-phase reactions, and hematopoiesis. IL-6 is produced by a variety of cells such as hepatocytes, B and T lymphocytes, neurons, tumor cells, and multi-potent hematopoietic cells that acts on a wide range of tissues. It plays a central role in the production of a variety of pathological conditions including inflammatory responses, sepsis, rheumatoid arthritis, allergy, trauma, pain, neurodegenerative diseases, as well as several other autoimmune diseases.
The Tribo™ Mouse IL-6 ELISA Kit is designed to detect mouse IL-6 levels quantitatively in different tissue including skin, muscle, neural, serum, and other biological samples. The plate has been coated with murine IL-6 monoclonal antibody and any IL-6 present in the sample or standard will be captured. A biotin-conjugated anti-murine IL-6 antibody is added along with streptavidin-horseradish peroxidase (HRP) accompanied by substrate. The reaction exhibits a blue color in direct proportion to the amount of IL-6 in the initial sample. The reaction can be terminated by the addition of a stop solution and absorbance is measured at 450nm. The concentration of IL-6 in the samples is calculated opposite to the standard curve.
1x 96-well plate (8x 12 strip well).
Direct Assays: quantitative IL-6 in different tissues.
- Sensitivity: The minimum detectable dose of mouse IL-6 is less than 8.0 pg/mL typically.
- Specificity: This assay recognizes recombinant and natural mouse IL-6. No cross-reactivity with any of other cytokine is observed.
|Precoated 96-well Plate||1|
|Standard||2x 100 μL|
|Detection A||1x 100 μL|
|Detection B||1x 100 μL|
|Sample Diluent (10x concentrate)||1x 12 ml|
|TMB Substrate Solution||1x 12 ml|
|Stop Solution||1x 12 ml|
|Wash Solution (20x concentrate)||1x 50 ml|
Store the kit at 4℃ and standard at -20℃, respectively.
Shelf life: 12 months after receiving.
1×96-well plate, 2×96-well plate