Fast Human Granzyme B ELISA; 1 step and 1hr.

Tribioscience’s Fast Human Granzyme B ELISA is designed to quantitatively detect human Granzyme B levels in serum, plasma, and other biological samples. It is a novel ELISA, different from the traditional ELISA approach. The kit uses our novel proprietary approaches to combine samples and detections into a one-step instead of the complicated traditional methods. It makes the assay simple, easy, accurate, and fast. The measurement can be finished in less than 2 hours, with no need for 4-5 hours (Fig. 1). The detection range is from 8 to 2000 pg/mL. The levels of human Granzyme B samples are parallel to the standard curves obtained using the kit standards linearly. Therefore, the kit can be used to determine relative mass values for natural human Granzyme B protein.

 

Typical Data

This standard curve(R2=0.996) is provided for demonstration only. A standard curve should be generated for each set of samples assayed. Fig. 3 is an example of typical Data.

 

Sensitivity

The minimum detectable dose (MOD) of human is typically 10 pg/ml.

The Intra-assay CV and the Inter-assay CV are ˂10%.

 

Specificity

This assay recognizes natural and recombinant human Granzyme B.

No cross-reactivity with others.

 

Simple Procedures

Simple Standard Preparation

Accurate Standard Curve

Kit Content and Storage Conditions

Part	Part#	Description	Storage of Opened/Reconstituted
Human Granzyme B Microplate	TBS3208A	96 well polystyrene microplate (12 strips of 8 wells) coated with a monoclonal antibody specific for human Granzyme.	Return unused wells to the foil pouch. Reseal along the entire edge of the zip-seal. May be stored for up to 1 month at 2-8 °C.
Human Granzyme B Standard	TBS3208B	30 µl of Recombinant human Granzyme B (100ng/mL).	Aliquot and store at -20 ℃ for up to 1 month in a manual defrost the freezer. Avoid repeated freeze-thaw cycles.
Detection A	TBS3208C	2.1 ml of Biotin-human Granzyme B antibody.	May be stored for up to 3 months at 2-8 °C.
Detection B	TBS3208D	12 ml of Streptavidin-HRP.
Assay Diluent	TBS3206E	12 ml of a buffered protein base with preservatives.
Wash Buffer	TBS3000W	12 mL of concentrated solution (10x).
TMB Substrate	TBS3000T	12 mL of ultra-sensitive TMB substrate.
Stop Solution	TBS3000S	6 mL of 2 N sulfuric acid.

Store the unopened kit at 2-8 ℃. Do not use past kit expiration dates.
The kit contains sufficient materials to run an ELISA on one 96 well plate.

 

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