Fast Human Granzyme B ELISA; 1 step and 1hr.
Cell culture Supernate, Plasma/Serum Granzyme B
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Tribioscience’s Fast Human Granzyme B ELISA is designed to quantitatively detect human Granzyme B levels in serum, plasma, and other biological samples. It is a novel ELISA, different from the traditional ELISA approach. The kit uses our novel proprietary approaches to combine samples and detections into a one-step instead of the complicated traditional methods. It makes the assay simple, easy, accurate, and fast. The measurement can be finished in less than 2 hours, with no need for 4-5 hours (Fig. 1). The detection range is from 8 to 2000 pg/mL. The levels of human Granzyme B samples are parallel to the standard curves obtained using the kit standards linearly. Therefore, the kit can be used to determine relative mass values for natural human Granzyme B protein.
This standard curve(R2=0.996) is provided for demonstration only. A standard curve should be generated for each set of samples assayed. Fig. 3 is an example of typical Data.
The minimum detectable dose (MOD) of human is typically 10 pg/ml.
The Intra-assay CV and the Inter-assay CV are ˂10%.
This assay recognizes natural and recombinant human Granzyme B.
No cross-reactivity with others.
Simple Standard Preparation
Accurate Standard Curve
Kit Content and Storage Conditions
|Part||Part#||Description||Storage of Opened/Reconstituted|
|Human Granzyme B Microplate||TBS3208A||96 well polystyrene microplate (12 strips of 8 wells) coated with a monoclonal antibody specific for human Granzyme.||Return unused wells to the foil pouch. Reseal along the entire edge of the zip-seal. May be stored for up to 1 month at 2-8 °C.|
|Human Granzyme B Standard||TBS3208B||30 µl of Recombinant human Granzyme B (100ng/mL).||Aliquot and store at -20 ℃ for up to 1 month in a manual defrost the freezer. Avoid repeated freeze-thaw cycles.|
|Detection A||TBS3208C||2.1 ml of Biotin-human Granzyme B antibody.||May be stored for up to 3 months at 2-8 °C.|
|Detection B||TBS3208D||12 ml of Streptavidin-HRP.|
|Assay Diluent||TBS3206E||12 ml of a buffered protein base with preservatives.|
|Wash Buffer||TBS3000W||12 mL of concentrated solution (10x).|
|TMB Substrate||TBS3000T||12 mL of ultra-sensitive TMB substrate.|
|Stop Solution||TBS3000S||6 mL of 2 N sulfuric acid.|
Store the unopened kit at 2-8 ℃. Do not use past kit expiration dates.
The kit contains sufficient materials to run an ELISA on one 96 well plate.