Fast Human Granzyme B ELISA; 1 step and 1hr.

Fast Human Granzyme B ELISA; 1 step and 1hr.


Cell culture Supernate, Plasma/Serum Granzyme B

SKU: N/A Category:
SKUStock SIZE PriceQuantity
TBS3208Yes1x96-well plate $460.00


Tribioscience’s Fast Human Granzyme B ELISA is designed to quantitatively detect human Granzyme B levels in serum, plasma, and other biological samples. It is a novel ELISA, different from the traditional ELISA approach. The kit uses our novel proprietary approaches to combine samples and detections into a one-step instead of the complicated traditional methods. It makes the assay simple, easy, accurate, and fast. The measurement can be finished in less than 2 hours, with no need for 4-5 hours (Fig. 1). The detection range is from 8 to 2000 pg/mL. The levels of human Granzyme B samples are parallel to the standard curves obtained using the kit standards linearly. Therefore, the kit can be used to determine relative mass values for natural human Granzyme B protein.


Typical Data

This standard curve(R2=0.996) is provided for demonstration only. A standard curve should be generated for each set of samples assayed. Fig. 3 is an example of typical Data.



The minimum detectable dose (MOD) of human is typically 10 pg/ml.

The Intra-assay CV and the Inter-assay CV are ˂10%.



This assay recognizes natural and recombinant human Granzyme B.

No cross-reactivity with others.


Simple Procedures


Simple Standard Preparation


Accurate Standard Curve


Kit Content and Storage Conditions

PartPart#DescriptionStorage of Opened/Reconstituted
Human Granzyme B MicroplateTBS3208A96 well polystyrene microplate (12 strips of 8 wells) coated with a monoclonal antibody specific for human Granzyme.Return unused wells to the foil pouch. Reseal along the entire edge of the zip-seal. May be stored for up to 1 month at 2-8 °C.
Human Granzyme B StandardTBS3208B30 µl of Recombinant human Granzyme B (100ng/mL).Aliquot and store at -20 ℃ for up to 1 month in a manual defrost the freezer. Avoid repeated freeze-thaw cycles.
Detection ATBS3208C2.1 ml of Biotin-human Granzyme B antibody.May be stored for up to 3 months at 2-8 °C.
Detection BTBS3208D12 ml of Streptavidin-HRP.
Assay DiluentTBS3206E12 ml of a buffered protein base with preservatives.
Wash BufferTBS3000W12 mL of concentrated solution (10x).
TMB SubstrateTBS3000T12 mL of ultra-sensitive TMB substrate.
Stop SolutionTBS3000S6 mL of 2 N sulfuric acid.

Store the unopened kit at 2-8 ℃. Do not use past kit expiration dates.
The kit contains sufficient materials to run an ELISA on one 96 well plate.


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Additional information


1×96-well plate


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