Pyruvate Colorimetric Assay (TBS2023C)

Description

Pyruvate is a central molecule in metabolic pathways. Pyruvate can be converted to carbohydrates via gluconeogenesis, to fatty acids or energy through acetyl-CoA, to the amino acid alanine and to ethanol. Abnormal levels of pyruvate have been linked to liver diseases and metabolic disorders.

Tribioscience’s Pyruvate Colorimetric Assay kit is designed to be a robust, simple and high throughput method in which pyruvate is oxidized to generate hydrogen peroxide. The resulting hydrogen peroxide is catalyzed and reacts with the probe measured by the colorimetric method at OD = 570 nm.

Assay Principle

Applications

Direct Assays: Detect as low as 4 µM of pyruvate in cells, serum and other biological samples.

Storage and Handling

Store kit at -20°C. Shelf life of 12 months.

Research use only.

Additional information

Datasheet:

TBS2023C Pyruvate colorimetric assay datasheet_V02_12-2023

MSDS:

TBS2023C Pyruvate Colorimetric Assay_MSDS

Research use only.