Caspase-3 Fluorometric Assay kit

Caspase-3 Fluorometric Assay kit

$289.00

Apoptosis plays a fundamental role in many normal biological processes as well as in several disease states. Apoptosis can be induced by various stimuli that all produce the same end result: systematic and orderly cell death. Caspases cleave a variety of cellular substrates after aspartic acid residues—a characteristic that is central to their role in mammalian apoptosis.

SKU: TBS2035 Category:
SKUStock SIZE (test) PriceQuantity
TBS2035Yes100 tests $289.00
spinner

Description

Apoptosis plays a fundamental role in many normal biological processes as well as in several disease states. Apoptosis can be induced by various stimuli that all produce the same result: systematic and orderly cell death.

Caspases cleave a variety of cellular substrates after aspartic acid residues – a characteristic that is central to their role in mammalian apoptosis. Caspases are synthesized in the cytosol of mammalian cells as inactive zymogens, which become active through intracellular caspase cascades.

The TribioTM Caspase-3 fluorometric Assay Kits provide a simple and convenient method for the activity analysis of caspases that recognize the sequence DEVD. The assay is based on the detection of the free AFC fluorescence after cleavage from the labeled substrate DEVD-AFC. The free AFC can be quantified using a fluorometer or a fluorescence microtiter plate reader at Ex400/Em505nm. Comparison of the fluorescence of AFC from an apoptotic sample with an uninduced control allows the determination of the fold increase in caspase-3 activity.

Applications

  • Apoptosis
  • Drug screening
  • Growth factors
  • Cytotoxicity

 

Related Products

  1. Resazurin Cell Viability (TBS2001)
  2. LDH Cytotoxicity Assay (TBS2002)
  3. MTT Cell Viability Assay (TBS 2003)
  4. MTS Cell Viability Assay (TBS2004)
  5. Catalase Assay (TBS2006)

 

Additional information

SIZE (test)

100 tests

Reviews

There are no reviews yet.


Be the first to review “Caspase-3 Fluorometric Assay kit”

You've just added this product to the cart: