Caspase-Family Colorimetric Assay Kit (TBS2050)

$589.00

Apoptosis plays a fundamental role in many normal biological processes as well as in several disease states. Apoptosis can be induced by various stimuli that all produce the same end result: systematic and orderly cell death.

SKU: TBS2050 Category:
SKUStock SIZE (test) PriceQuantity
TBS2050 Yes 9x25 Tests $589.00
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Description

Histones are the chief protein components of chromatin. They act as spools around which DNA winds. Covalent modification of histone proteins through acetylation and deacetylation affects chromatin structure and regulates gene expression. Histone hyperacetylation is well correlated with increased transcription, whereas hypoacetylation correlates with transcriptional repression. Histone deacetylases (HDACs) catalyze the removal of acetyl groups from an ɛ-N-acetyl lysine amino acid on a histone, and act as transcriptional repressors of genes. Histone deacetylases have been grouped into three classes. Class I (HDAC 1, 2, 3, 8) and Class II (HDAC 4, 5, 6, 7, and 9) are zinc-containing hydrolase enzymes. Class III consists of the members of the sirtuin family of enzymes (Sir 1 to 7). Inhibitors of HDAC classes I and II are being studied as a treatment for cancer and neurodegenerative diseases such as Huntington’s and Alzheimer’s diseases. The Sirtuin 1 (class III) enzyme represents a target for the treatment of age-related diseases and type II diabetes.

The TriboTM HDAC Activity Assay Kit provides a fast, accurate, and simple approach for DAC activity detection from various sources of enzyme. The kit uses a fluorogenic substrate for HDAC activity detection. An acetylated substrate is incubated with HDAC-containing samples. Deacetylation of the substrate in the presence of the HDAC developer generates strong fluorescence, which can be detected at Ex354nm/Em442nm. The substrate included in the kit is cell-permeable, and the assay can measure HDAC activity directly in cell culture in a 96-well plate without a time-consuming cell extraction step. The kit also can be used for HTS of HDAC inhibitors with extracts or purified enzymes.

 

Storage Conditions

Store the substrate at –20°C protected from light. The other components may be stored at 4°C. Shelf life: 12 months.

Additional information

SIZE (test)

9×25 Tests

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