Pyruvate Fluorometric Assay (TBS2023F)

Description

Pyruvate is a central molecule in metabolic pathways. Pyruvate can be converted to carbohydrates via gluconeogenesis, to fatty acids or energy through acetyl-CoA, to the amino acid alanine and to ethanol. Abnormal levels of pyruvate have been linked to liver diseases and metabolic disorders.

Tribioscience’s Pyruvate Fluorometric Assay kit is designed to be a robust, simple, and high throughput method in which pyruvate is oxidized to generate hydrogen peroxide. The resulting hydrogen peroxide is catalyzed and reacts with the probe measured by the fluorometric method at Ex/Em=530/590 nm.

Assay Principle

Applications

Direct Assays: Detect as low as 1 µM of pyruvate in cells, serum, and other biological samples.

Storage and Handling

Store kit at -20°C. Shelf life of 12 months.

Research use only.

Additional information

Datasheet:

TBS2023F_Pyruvate fluorometric assay datasheet_V02_12-2023

MSDS:

TBS2023F Pyruvate Fluorometric Assay_MSDS

Research use only.