Tribo™ Tryptase Activity Assay

Tribo™ Tryptase Activity Assay

$280.00

The Tribo™ Tryptase Activity Assay Kit provides a quick, efficient, and sensitive system of evaluation of tryptase activity in cell lysates and supernatants as well as inhibitor screening.

SKU: TBS2101 Category:
SKUStock SIZE (test) PriceQuantity
TBS2101Yes100 tests $280.00
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Description

Tryptase, a tetrameric serine proteinase, has emerged as the major component of mast cell granules. Tryptase comprises up to 20% of the total protein in mast cells derived from lung, colon, and skin tissue. Because it is stored almost exclusively in mast cells, tryptase is a popular indicator of mast cell activation and a target for therapeutic intervention in allergic diseases.
The Tribo™ Tryptase Activity Assay Kit provides a quick, efficient, and sensitive system for evaluating tryptase activity in cell lysates and supernatants and can be used in inhibitor screening.

Applications:

  • Cell culture supernatants, cell lysates, or other tryptase containing supplies.
  • Testing of purified tryptase enzyme, in vitro inhibitor screening.
  • Cell degranulation

 

Key Features:

  • Sensitive and Accurate: The kit detects as low as 10μM in solution.
  • Simple and High-Throughput: The procedure is easily adapted to automation with no separation required as a high-throughput assay.

 

Kit Contents:

(for 100x assays)

ComponentAmount
5x Assay Buffer20 mL
Tryptase inhibitor200μL (10mM)
Tryptase Substrate2mL (2.5mM)
pNA Standard100μL (10mM)
Calcium Ionophore50μL (1mM)
Tryptase Positive Control200μL (10 μg/mL)

 

Storage Conditions:

Store the kit at -20℃ protected from life
Shelf life: 6 months.

 

Example of pNA Standard Curve

Example Tryptase Positive Control Curve

 

Related Products:

  1. β-Hexosaminidase Activity Assay (TBS2105)
  2. Cytochrome C Oxidase Activity Assay (TBS2115)
  3. Fast Glucose Determination Assay (TBS2087)

 

Additional information

SIZE (test)

100 tests

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