Cystathionine-β-lyase (CBL) enzyme (TBP0224)
$79.00 – $199.00
| SKU | Stock | SIZE | Price | Quantity | ||
|---|---|---|---|---|---|---|
| TBP0224-500 | Yes | 500 U | $79.00 | |||
| TBP0224-2000 | Yes | 2000 U | $199.00 |
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Description
Cystathionine-β-Lyase (CβL) is a pyridoxal phosphate (PLP)-dependent enzyme that catalyzes the cleavage of L-cystathionine into L-cysteine, α-ketobutyrate, and ammonia. This enzyme plays a key role in sulfur amino acid metabolism, particularly in the biosynthesis of cysteine from homocysteine.
Cystathionine-β-lyase (EC 4.4.1.8, from Microorganism)
L-Cystathionine —-> L-Homocysteine + Pyruvate + NH3
Specifications
| Appearance: | Yellow amorphous powder, lyophilized | |
| Purity: | ≥90.0% | |
| Enzyme powder specific activity: | ≥30.0 U/mg | |
| Glucose-6-phosphate dehydrogenase: | ≤0.01% | |
| Lactate dehydrogenase: | ≤0.01% | |
| EC number: | 4.4.1.8 (Recombinant from microorganism) | |
| Molecular weight: | 54 kDa | |
| Isoelectric point: | 6 | |
| Michaelis constants: | 1.6 ×10-3 M (L-Cystathionine) | |
| Inhibitors: | Not inhibited by NaN3 | |
| Optimum pH: | 8 | Fig. 1 |
| Optimum temperature: | 50 ℃ | Fig. 2 |
| pH stability: | pH 6.0-10.0 (25 ℃, 16 h) | Fig. 3 |
| Thermal stability: | Below 45℃ (pH 8.0, 30 min) | Fig. 4 |
| Storage stability: | At least one year at -25～-15 ℃ | Fig. 5 |
| Stabilizers: | Triton X-100, glycerol | |
| Unit definition:
|
One unit (U) is defined as the amount of enzyme which consumes 1 μmol of NADH per min under the conditions described below. | |
Applications
This enzyme is useful for enzymatic determination of L-homocysteine when coupled with CBS and LDH in clinical analysis.
Fig. 1 Optimum pH Buffer solution: pH 4.5-5.5, acetate buffer; pH 6.0-8.0, Na-phosphate; pH 8.0-9.0, Tris-HCl; pH 9.0-10.0, Glycine-NaOH. Enzyme concentration: 1 mg/mL |
Fig. 2 Optimum temperature Reaction in 50 mM Tris-HCl buffer, pH 8.0. Enzyme concentration: 1 mg/mL |
Fig. 3 pH Stability 25℃, 16 h-treatment with 50 mM buffer solution: pH 4.5-5.5, acetate buffer; pH 6.0-8.0, Na-phosphate; pH 8.0-9.0, Tris-HCl; pH 9.0-10.0, Glycine-NaOH. Enzyme concentration: 1 mg/mL |
Fig 4 Thermal stability 30 min-treatment with 50 mM Tris-HCl buffer, pH 8.0. |
Fig.5 Storage stability (-25～-15 ℃) |
Research use only.
Additional information
| SIZE | 2000 U, 500 U |
|---|
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