Cystathionine β-Synthase (TBP0223)
$199.00 – $499.00
| SKU | Stock | SIZE | Price | Quantity | ||
|---|---|---|---|---|---|---|
| TBP0223-500 | Yes | 500 U | $199.00 | |||
| TBP0223-2000 | Yes | 2000 U | $499.00 |
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Description
Cystathionine β-Synthase (CBS) is a key enzyme in the transsulfuration pathway that catalyzes the conversion of homocysteine and serine into cystathionine in a pyridoxal-5′-phosphate (PLP)–dependent manner. It plays an essential role in homocysteine metabolism, cysteine biosynthesis, and the production of the gasotransmitter hydrogen sulfide (H₂S). Dysregulation of CBS is associated with homocystinuria, cardiovascular disorders, and neurological diseases, making it an important target in metabolic research and drug discovery.
Cystathionine-β-synthase (EC 4.2.1.22, from Microorganism)
L-Homocysteine + L-Serine —–> L-Cystathionine + H2O
Specifications
| Appearance: | Yellow amorphous powder, lyophilized | |
| Protein purity: | ≥90% | |
| Activity | ≥8 U/mg solid | |
| Glucose-6-phosphate dehydrogenase: | ≤0.01% | |
| Lactate dehydrogenase: | ≤0.01% | |
| EC number: | 4.2.1.22 (Recombinant from microorganism) | |
| Molecular weight: | 51 kDa | |
| Isoelectric point: | 5.4 | |
| Michaelis constants: | 8.0 ×10-4 M (L-Serine), 1.6 ×10-4 M (L-Homocysteine) | |
| Inhibitors: | Not inhibited by NaN3 | |
| Optimum Ph: | 8 | Fig. 1 |
| Optimum temperature: | 40 ℃ | Fig. 2 |
| pH stability: | pH 6.0-10.0 (25℃, 16 h) | Fig. 3 |
| Thermal stability: | Below 45 ℃ (pH 8.0, 30 min) | Fig. 4 |
| Storage stability: | At least one year at -25～-15 ℃ | Fig. 5 |
| Stabilizers: | Triton X-100 | |
Applications
This enzyme is useful for enzymatic determination of L-homocysteine when coupled with CBS and LDH in clinical analysis.
Fig. 1 Optimum pH Buffer solution: pH 5.0-6.0, Acetate; pH 6.0-7.5, K-phosphate; pH 7.5-9.0, Tris-HCl; pH 9.0-10.0, Glycine-NaOH. Enzyme concentration: 1 mg/mL |
Fig. 2 Optimum temperature Reaction in 50 mM Na-phosphate buffer, pH 8.0. Enzyme concentration: 1 mg/mL |
Fig. 3 pH Stability 25 ℃, 16 h-treatment with 50 mM buffer solution: pH 4.5-5.5, Acetate; pH 6.0-8.0, K-phosphate; pH 8.0-9.0, Tris-HCl; pH 9.0-10.0, Glycine-NaOH. Enzyme concentration: 1 mg/mL |
Fig. 4 Thermal stability 30 min-treatment with 50 mM Na-phosphate buffer, pH 8.0. Enzyme concentration: 1 mg/mL |
Fig.5 Storage stability (-25～-15 ℃)
Research use only.
Additional information
| SIZE | 2000 U, 500 U |
|---|
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