Human Adenosylhomocysteinase / AHCY Recombinant Protein, Bioactive (TBP0003)

$210.00$1,875.00

SKU: TBP0003 Category:
SKUStock SIZE PriceQuantity
TBP0003-20UG Yes 20 µg $210.00
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TBP0003-100UG Yes 100 µg $630.00
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TBP0003-500UG Yes 500 µg $1,875.00
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Description

Adenosylhomocysteinase (AHCY) is an enzyme that catalyzes the reversible hydrolysis of S-adenosylhomocysteine (SAH) to adenosine (Ado) and L-homocysteine (Hcy). It regulates the intracellular SAH concentration thought to be important for transmethylation reactions. Deficiency in this protein is one of the different causes of hypermethioninemia. Recombinant human AHCY protein, fused to His-tag at N-terminus, was expressed in E. coli, and purified by using proprietary chromatography techniques. This enzyme is validated for AHCY activity application.

 

Product Details

Expression System: E. coli.

Domain: 1-432aa.

UniProt No: P23526.

Alternative Names: Adenosyl homocysteinase, AHCY, AdoHcyase, SAHH, S-adenosylhomocysteine hydrolase.

 

Product Specification

Molecular Weight: 49.8 kDa (452aa) confirmed by MALDI-TOF

Concentration: 1mg/ml (determined by Bradford assay).

Formulation: Liquid in Phosphate-Buffered Saline (pH 7.4) containing 20% glycerol, No DTT.

Purity: > 95% by SDS-PAGE.

Tag: His-Tag.

Biological Activity: Specific activity is > 300pmol/min/mg.

Storage Condition: Store at -20℃ to -80℃. Avoid repeated freezing and thawing cycles.

 

Applications

  • Enzyme Activity, and protein assay by SDS-PAGE

Additional information

SIZE

100 µg, 20 µg, 500 µg

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