Glucose-6-phosphate dehydrogenase, Enzyme Activity (TBP0017)

Description

Preparation and Specification

Appearance: White amorphous powder, lyophilized

Activity: GradeⅢ 200U/mg-solid or more

Contaminants: Creatine phosphokinase ≤1×10⁻³%

Phosphoglucomutase ≤1×10⁻³%

6-Phosphogluconate dehydrogenase ≤5×10⁻³%

Phosphoglucose isomerase ≤1×10⁻²%

Glutathione reductase ≤1×10⁻³%

Hexokinase ≤1×10⁻²%

Myokinase ≤1×10⁻²%

NADH oxidase ≤1×10⁻²%

NADPH oxidase ≤1×10⁻²%

Properties

Stability: Stable at -20°C for at least One year

Molecular weight: approx. 140,000 (by gel filtration)

Michaelis constants: NAD⁺ linked 2.4×10⁻⁴M (NAD⁺), 4.7×10⁻⁴M (G-6-P)

NADP⁺ linked 7.4×10⁻⁶M (NADP⁺), 3.2×10⁻⁴M (G-6-P)

Inhibitors: Metal ions, iodoacetamimide, SDS etc.

Optimum pH: 7.8

Optimum temperature: 50°C-55°C

pH Stability: pH 5.0-11.0 (25°C, 22hr)

Thermal stability: below 50°C (pH 7.8, 30min)

Applications

The enzyme is useful for enzymatic determination of NAD⁺(NADP⁺) and G-6-P, and activities of phosphoglucose isomerase, phosphoglucomutase and hexokinase. The enzyme is also used for enzymatic determination of glucose and creatine phosphokinase activity when coupled with hexokinase.

Research use only.

Additional information

Datasheet:

TBP0017-Glucose-6-phosphate dehydrogenase

MSDS:

TBP0017_Glucose-6-phosphate dehydrogenase Safety Data Sheet

Research use only.