Cyclic GMP Colorimetric Activity Assay (TBS2074)
$490.00
SKU | Stock | SIZE (test) | Price | Quantity | ||
---|---|---|---|---|---|---|
TBS2074-100 | Yes | 100 Tests | $490.00 |
- Description
- Terms
- Additional information
- Documents
- Reviews (0)
Description
This assay kit is a competition enzyme-linked immunoassay designed to measure cGMP levels in cells or tissues. An anti-rabbit IgG was pre-coated onto a microplate. The cGMP in test sample competes with cGMP-HRP for binding to an anti-cGMP rabbit monoclonal antibody. Following the incubation and wash step, an ultra-sensitive substrate TMB is added for color development. Because of the competitive nature of this assay, the color intensity is inversely proportional to the quantity of sample cGMP levels. Measurement of absorbance using the cGMP Standard allows calculating the absolute amount of cGMP in a sample. This assay provides a flexible, accurate, simple, and time-saving approach for cGMP detection.
Typical Data
This standard curve is provided for demonstration only. A standard curve should be generated for each set of samples assayed. Fig. 2 is an example of typical Data.
Sensitivity
The minimum detectable dose (MOD) of cGMP is typically 1 pM.
The Intra-assay CV and the Inter-assay CV are ˂10%.
Specificity
This assay recognizes cGMP. No cross-reactivity with others.
Standard Preparation
Standard Curve
Kit Content and Storage Conditions
Part | Part# | Description | Storage of Opened/Reconstituted |
---|---|---|---|
Precoated Microplate | TBS2074A | 96 well polystyrene microplate (12 strips of 8 wells) coated with anti-mouse IgG antibody. | Return unused wells to the foil pouch. Reseal along the entire edge of the zip-seal. May be stored for up to 1 month at 2-8 °C. |
cGMP Standard | TBS2074B | 50 µL of cGMP (1 µM). | Aliquot and store at -20 ℃ for up to 1 month in a manual defrost freezer. Avoid repeated freeze-thaw cycles. |
Detection A | TBS2074C | 110 µL of cGMP-HRP (25x). | May be stored for up to 3 months at 2-8 °C. |
Detection B | TBS2074D | 1.1 mL of anti-cGMP antibody (5x). | |
Assay Diluent | TBS2074E | 20 mL of a buffered protein base with preservatives. | |
Wash Buffer | TBS3000W | 12 mL of concentrated solution (10x). | |
TMB Substrate | TBS3000T | 12 mL of ultra-sensitive TMB substrate. | |
Stop Solution | TBS3000S | 6 mL of 2 N sulfuric acid. |
Store the unopened kit at 2-8 ℃. Do not use past kit expiration dates.
The kit contains sufficient materials to run an ELISA on one 96-well plate.
Additional information
SIZE (test) | 100 Tests |
---|
Be the first to review “Cyclic GMP Colorimetric Activity Assay (TBS2074)”
You must be logged in to post a review.
Reviews
There are no reviews yet.