Acid Phosphatase Activity Colorimetric Assay (TBS2075)

$390.00$690.00

SKU: TBS2075 Categories: ,
SKUStock SIZE (test) PriceQuantity
TBS2075-100 Yes 100 Tests $390.00
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TBS2075-200 Yes 200 Tests $690.00
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Description

Acid phosphatases (AP) dephosphorylate phosphate groups from phosphate esters under acid conditions. Different acid phosphatase isozymes are found in different organs, and their serum levels are used as a diagnostic index for disease in the corresponding organs. Elevated prostatic acid phosphatase levels may indicate the presence of prostate cancer and elevated tartrate-resistant acid phosphatase levels may indicate bone disease.

Tribioscience’s Acid Phosphatase Colorimetric Assay Kit provides a high-sensitive, simple, and direct assay approach to measure AP activity in serum and other samples. It is suitable for research and drug discovery. The kit uses p-nitrophenyl phosphate (pNPP) as a phosphatase substrate which turns yellow (λmax = 405 nm) when dephosphorylated by AP. The kit can detect as low as 20 µU acid phosphatase activity in samples.

 

Applications

Direct Assays: Acid phosphatase in serum, plasma, urine, and other bio-samples.

 

Key Features

  • Flexible: Suitable for colorimetric assay.
  • Accurate: Use 50 µL samples. Detection ranges from 0.4-200 µU in a 96-well plate for colorimetric assay.
  • Simple and high-throughput: Just load-incubate-Read. The kit can be used for a robust method.
  • Time-saving: less than 30 minutes.

 

Standard Curve Preparations

 

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Additional information

SIZE (test)

100 Tests, 200 Tests

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