CD203a Activity Assay (TBS2113)

$560.00

Synonyms: ENPP1; PC-1; ARHR2; COLED; M6S1; PDNP1

SKU: TBS2113 Category:
SKUStock SIZE (test) PriceQuantity
TBS2113 Yes 100 Assays $560.00
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Description

CD203a (ENPP1) is a key well-known cell surface marker and enzyme which hydrolyzes ATP, cGAMP and other substrates. It plays roles in purinergic signaling, bone and soft tissue mineralization, immune regulation, cGAS/STING pathway modulation and tumor microenvironment regulation. It is very important in both basic research and clinical applications.

 

Tribioscience CD203a Activity Colorimetric Assay provides a simple and sensitive method for monitoring CD203a activity in biological samples (cells, tissue, serum, urine, stool). This assay uses a synthetic CD26 substrate to release p-nitrophenol (pNP), which turns yellow under alkaline conditions. The absorbance of pNP is measured at 405 nm as shown in Fig. 1.

 

Fig. 1: Assay Principle

 

 

APPLICATIONS

Determination of CD203a activity in biological samples.

 

KEY FEATURES

Flexible: can be used for 96 wells and 384 wells plate.

Simple: Just one-step: add-incubate-read model.

Time saving: a 30-minute reaction at 37˚C

 

KIT CONTENTS

Component 100x Rxns
CD203a Substrate 0.45 mL
pNP Standard (10mM) 0.1 mL
Positive control 5 µL
Assay Buffer 12 mL
Activator buffer 1 mL

 

STORAGE CONDITIONS

The kit is shipped on ice and should be stored at -20°C for shelf life of 12 months after receipt.

Research use only.

Additional information

SIZE (test)

100 Assays

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