Cyclic AMP Colorimetric Assay (TBS2073)
$490.00
SKU | Stock | SIZE (test) | Price | Quantity | ||
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TBS2073-100 | Yes | 100 Tests | $490.00 |
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Description
This assay kit is a competition enzyme-linked immunoassay designed to measure cAMP levels in cells or tissues. An anti-mouse IgG was pre-coated onto a microplate. The cAMP in the test sample competes with cAMP-HRP for binding to an anti-cAMP mouse monoclonal antibody. Following the incubation and wash step, an ultra-sensitive substrate TMB is added for color development. Because of the competitive nature of this assay, the color intensity is inversely proportional to the quantity of sample cAMP levels. Measurement of absorbance using the cAMP Standard allows calculating the absolute amount of cAMP in a sample. This assay provides a flexible, accurate, simple, and time-saving approach for cAMP detection.
Typical Data
This standard curve is provided for demonstration only. A standard curve should be generated for each set of samples assayed. Fig. 2 is an example of typical Data.
Sensitivity
The minimum detectable dose (MOD) of cAMP is typically 1 nM.
The Intra-assay CV and the Inter-assay CV are ˂10%.
Specificity
This assay recognizes cAMP. No cross-reactivity with others.
Standard Preparation
Standard Curve (Fig.2)
Kit Content and Storage Conditions
Part | Part# | Description | Storage of Opened/Reconstituted |
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Precoated Microplate | TBS2073A | 96 well polystyrene microplate (12 strips of 8 wells) coated with anti-mouse IgG antibody. | Return unused wells to the foil pouch. Reseal along the entire edge of the zip-seal. May be stored for up to 1 month at 2-8 °C. |
cAMP Standard | TBS2073B | 50 µL of cAMP (1 mM). | Aliquot and store at -20 ℃ for up to 1 month in a manual defrost freezer. Avoid repeated freeze-thaw cycles. |
Detection A | TBS2073C | 110 µL of cAMP-HRP (25x). | May be stored for up to 3 months at 2-8 °C. |
Detection B | TBS2073D | 1.1 mL of anti-cAMP antibody (5x). | |
Assay Diluent | TBS2073E | 20 mL of a buffered protein base with preservatives. | |
Wash Buffer | TBS3000W | 12 mL of concentrated solution (10x). | |
TMB Substrate | TBS3000T | 12 mL of ultra-sensitive TMB substrate. | |
Stop Solution | TBS3000S | 6 mL of 2 N sulfuric acid. |
Store the unopened kit at 2-8 ℃. Do not use past kit expiration dates.
The kit contains sufficient materials to run an ELISA on one 96-well plate.
Additional information
SIZE (test) | 100 Tests |
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