ATP Colorimetric/Fluorometric Assay Kit

ATP Colorimetric/Fluorometric Assay Kit

$350.00

Tribio™ ATP Colorimetric and Fluorometric Assay kit is designed to be a robust, simple method which utilizes the phosphorylation of glycerol to generate a product that is easily quantified by colorimetric (OD = 570 nm) or fluorometric (Ex/Em = 535/590 nm) methods.

SKU: TBS2010 Category:
SKUStock SIZE (test) PriceQuantity
TBS2010Yes100 tests $350.00
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Description

Adenosine-5′-triphosphate (ATP) is a multifunctional nucleotide used in cells as a coenzyme. ATP is the main energy source for a majority of cellular functions. ATP is also critical in maintaining cell structure by facilitating assembly and disassembly of elements of the cytoskeleton. Tribioscience’s ATP Colorimetric and Fluorometric Assay kit is designed to be a robust, simple method which utilizes the phosphorylation of glycerol to generate a product that is easily quantified by colorimetric (OD = 570 nm) or fluorometric (Ex/Em = 535/590 nm) methods. The assay can detect down to 0.5 µM of ATP in various samples.

Applications:

Direct Assays as low as 0.5 µM of ATP in cells and other biological samples can be conducted.
Assay of enzymes that produce or degrade ATP.

Key Features:

Sensitive and accurate: Use 10 μL samples. Detection range 0.5-1000µM in 96-well plate assay.
Simple and high-throughput: Simple procedure; takes less than 30 minutes. Kit is designed to be a robust method.

Kit Contents:

Storage Conditions:Store all components at –20℃ Shelf Life – 3 months
Warm all components at room temperature prior to use except enzyme.

Assay Buffer24mL
Probe120μL
Substrate120μL
Enzyme240μL
ATP Standard (50mM)100μL

Additional information

SIZE (test)

100 tests

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