Ketoamine Oxidase (TBP0221)

Description

Ketoamine Oxidase is a highly specific oxidative enzyme that catalyzes the conversion of ketoamine substrates with excellent activity and selectivity. Its robust stability, broad substrate tolerance, and compatibility with mild reaction conditions make it a valuable tool for biochemical studies, pathway elucidation, and biocatalytic method development. The enzyme supports applications in metabolic research, analytical assays, and the synthesis of structurally defined intermediates.

Ketoamine oxidase (EC 1.5.3, from Microorganism)

Fructosyl-L-amino acid + H₂O + O₂     —–>       L-Amino acid + Glucosone + H₂O₂

Specifications

Applications

The enzyme is useful for the determination of fructosyl-L-amino acid.

Fig. 1 Optimum pH Buffer solution: pH 5.2-5.8, acetate buffer; pH 6.2-8.2, Na-phosphate; pH 8.2-9.2, Tris-HCl. Enzyme concentration: 1 mg/mL	Fig. 3 pH Stability 25 ℃, 16 h-treatment with 50 mM buffer solution: pH 4.5-6.0, acetate buffer; pH 6.5-8.0, Na-phosphate; pH 8.0-9.0, Tris-HCl; pH 9.0-10.0, Glycine-NaOH. Enzyme concentration: 1 mg/mL
Fig. 2 Optimum temperature Reaction in 20 mM Tris-HCl buffer, pH 8.0. Enzyme concentration: 1 mg/mL	Fig. 4 Thermal stability 30 min-treatment with 100 mM phosphate buffer, pH 8.0 Enzyme concentration: 1 mg/mL
Fig.5 Storage stability (-25～-15 ℃)

Research use only.

Additional information

Datasheet:

TBP0221 KAOD Ketoamine oxidase_Datasheet_v1

MSDS:

TBP0221 KAOD Ketoamine oxidase MSDS_v01

Research use only.