Proline specific endopeptidase, Enzyme Activity (TBP0040)

$332.00$530.00

SKU: TBP0040 Category:
SKUStock SIZE PriceQuantity
TBP0040-50U Yes 50 U $332.00
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TBP0040-100U Yes 100 U $530.00
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Description

Preparation and Specification

Appearance: White amorphous powder, lyophilized

Activity: GradeⅠ 5.0U/mg-solid or more

Contaminants: Leucine aminopeptidase ≤1.0×10⁻¹%

Trypsin-like activity ≤1.0×10⁻¹%

 

Properties

Stability: Stable at -20°C for at least One year

Molecular weight: approx. 78,000

Isoelectric point: 9.1

Michaelis constants: 2.5×10⁻⁵M (Z-Gly-Pro-MCA), 1.4×10⁻⁴M (Z-Gly-Pro-2NNap)

Structure: Monomer

Inhibitors: DFP, 3, 4-dichloroisocoumarin, Z-Gly-Pro-CH₂Cl

Optimum pH: 6.5

Optimum temperature: 37°C (40°C)

pH Stability: 5.5-8.5 (30°C,15hr)

Thermal stability: below 40°C (pH7.0,10min)

 

Applications

This enzyme is useful for the determination of amino acid sequences of peptides and proteins containing proline residues.

Additional information

SIZE

50 U, 100 U

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