Cholesterol oxidase, Enzyme Activity (TBP0013)

$429.00$686.00

SKU: TBP0013 Category:
SKUStock SIZE PriceQuantity
TBP0013-500U Yes 500 U $429.00
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TBP0013-1KU Yes 1000 U $686.00
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Description

Preparation and Specification

Appearance: Yellowish amorphous powder, lyophilized

Activity: GradeⅢ 12U/mg-solid or more

Contaminants: Catalase ≤1.0×10-1%, Cholesterol esterase ≤1.0×10-2%

Stabilizers: BSA, amino acids

 

Properties

Stability: Stable at −20℃ for at least one year

Molecular weight: approx. 55,000 (by gel-filtration)

Isoelectric point: 4.6±0.1, 4.9±0.1 and 5.2±0.1

Michaelis constant: 2.1×10-5M (Cholesterol)

Inhibitors: Ionic detergents, Ag, Hg++

Optimum pH: 7.0−8.0

Optimum temperature: 60℃

pH Stability: pH 5.0−10.0 (25℃, 20hr)

Thermal stability: below 55℃ (pH 7.0, 15min)

 

Applications

This enzyme is useful for enzymatic determination of cholesterol in serum when coupled with cholesterol

esterase (COE-301, COE-311, COE-313) in clinical analysis.

Additional information

SIZE

1000 U, 500 U

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