Ultra-Sensitive Fast Human TGF-β1 ELISA Kit

$450.00

Ultra-sensitive Human TGF-β1 ELISA Kit, Detect as low as 0.5pg / mL

SKU: TBS3232U Categories: ,
SKUStock SIZE (kit) PriceQuantity
TBS3232U Yes 1 Kit $450.00
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Description

The Ultra-sensitive Human TGF-β1 ELISA is a solid phase ELISA to measure the low concentration of human TGF-β1 at 0.5pg/mL level in cell culture supernatants, serum, and plasma, which is not possible to be detected with the regular ELISA. The main feature of this kit is the use of a novel approach to replace traditional methods. The kit is designed to ensure short steps, a short incubation time, and only one-step washing out. The measurement can be finished within 2 hours instead of the traditional 4-5 hours. The detection range is between 0.5-2048 pg/mL. The levels of human TGF-β1 samples are parallel to the standard curves obtained using the kit standards linearly. These results indicate that this kit can be used to determine relative mass values for natural human TGF-β1 protein.

 

Simple Procedures

 

Simple Standard Preparation

 

Accurate Standard Curve

 

Kit Contents and Storage Conditions

Part Part# Description Storage of Opened/Reconstituted
Human TGF-β1 Microplate TBS3232UA 96-well microplate (12-strips of 8-wells) coated with a Capture Antibody specific for human TGF-β1. The unused wells can be stored in the sealed foil pouch containing the desiccant pack for up to 1 month at 2-8 °C.
Human TGF-β1 Standard TBS3232UB 25 µL of Recombinant human TGF-β1protein (102.4 ng/mL). Aliquot and store at -20 ℃ for up to 6 months in a manual defrost freezer. Avoid repeated freeze-thaw cycles.
Detection A TBS3232UC 2.2 mL of human TGF-β1 antibody. May be stored for up to 3 months at 2-8℃
Detection B TBS3232UD 12 mL of Streptavidin-HRP (1x)
Assay Diluent TBS3232UE 12 mL of buffered protein base with preservatives.
10x Wash Buffer TBS3000W 12 mL of concentrated solution (10x).
TMB Substrate TBS3000T 12 mL of ultra-sensitive TMB substrate.
Stop Solution TBS3000S 6 mL of 2 N sulfuric acid.

Store the unopened kit at 2-8℃. Do not use past kit expiration dates.
The kit contains sufficient materials to run an ELISA on one 96-well plate.

 

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Additional information

SIZE (kit)

1 Kit

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