Ultra-Sensitive Fast Human TGF-β1 ELISA Kit
Ultra-sensitive Human TGF-β1 ELISA Kit, Detect as low as 0.5pg / mL
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Transforming Growth Factor Β (TGF-β) is a stable, multifunctional polypeptide growth factor. TGF-β exists in at least five unique isoforms; TGF-β1, TGF-β2, TGF- β3, TGF-β4, and TGF-β5. TGF-β1 is the prevalent form and is found almost ubiquitously while the other isoforms are expressed in a more limited spectrum of cells and tissues. It is normally secreted as an inactive or latent complex.
The Ultra-sensitive Human TGF-β1 ELISA is a solid phase ELISA to measure the low concentration of human TGF-β1 at 0.5pg/mL level in cell culture supernatants, serum, and plasma, which is not possible to be detected with the regular ELISA. The main feature of this kit is the use of a novel approach to replace traditional methods. The kit is designed to ensure short steps, a short incubation time, and only one-step washing out. The measurement can be finished within 2 hours instead of the traditional 4-5 hours. The detection range is between 0.5-2048 pg/mL. The levels of human TGF-β1 samples are parallel to the standard curves obtained using the kit standards linearly. These results indicate that this kit can be used to determine relative mass values for natural human TGF-β1 protein.
Principle of the Assay:
This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for human TGF-β1 was pre-coated onto a microplate. Standards and samples are then pipetted into the wells and incubated with a detection antibody specific for human TGF-β1. Aspirate each well, then add Streptavidin-HRP, incubate the complex. Following a wash to remove any unbound antibodies and samples, an ultra-sensitive TMB substrate solution is added to the wells for color development. The color intensity is proportional to the amount of TGF-β1 bound in the initial step. The intensity of the color is measured by plate read at 450 nm.
Kit Contents and Storage Conditions:
|Part||Part#||Description||Storage of Opened/Reconstituted|
|Human TGF-β1 Microplate||TBS3232UA||96-well microplate (12-strips of 8-wells) coated with a Capture Antibody specific for human TGF-β1.||The unused wells can be stored in the sealed foil pouch containing the desiccant pack for up to 1 month at 2-8 °C.|
|Human TGF-β1 Standard||TBS3232UB||25 µL of Recombinant human TGF-β1protein (102.4 ng/mL).||Aliquot and store at -20 ℃ for up to 6 months in a manual defrost the freezer. Avoid repeated freeze-thaw cycles.|
|Detection A||TBS3232UC||2.2 mL of human TGF-β1 antibody.||May be stored for up to 3 months at 2-8℃|
|Detection B||TBS3232UD||12 mL of Streptavidin-HRP (1x)|
|Assay Diluent||TBS3232UE||12 mL of buffered protein base with preservatives.|
|10x Wash Buffer||TBS3000W||12 mL of concentrated solution (10x).|
|TMB Substrate||TBS3000T||12 mL of ultra-sensitive TMB substrate.|
|Stop Solution||TBS3000S||6 mL of 2 N sulfuric acid.|
Store the unopened kit at 2-8℃. Do not use past kit expiration dates.
The kit contains sufficient materials to run an ELISA on one 96-well plate.